Hai Xunan, Zhang Houshuang, Wang Zhonghua, Gong Haiyan, Cao Jie, Zhou Yongzhi, Zhou Jinlin
Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
Front Microbiol. 2017 Oct 10;8:1959. doi: 10.3389/fmicb.2017.01959. eCollection 2017.
Peroxiredoxins (Prxs) are a family of antioxidant enzymes that reduce peroxides in the presence of thioredoxin, thioredoxin reductase, and nicotinamide adenine dinucleotide phosphate (NADPH) to resist oxidative stress. In this study, we identified and isolated a 2-Cys Prx designated as '' from , with a full-length cDNA of 826 bp and an open reading frame of 756 bp, which encodes a 251-amino acid protein. BLAST analysis demonstrated that BmTPx-2 shows the typical features of members of the 2-Cys Prx family, which includes harboring two conserved VCP motifs with Cys and Cys conserved cysteine residues. Recombinant BmTPx-2 was expressed in and analyzed by western blot. The antioxidant activity of BmTPx-2 was demonstrated using a mixed-function oxidation system and oxidation of NADPH. Furthermore, mRNA expression level in parasites at the erythrocytes and tick stages were analyzed by real-time fluorescence quantitative PCR. Peak mRNA transcription was detected 8 days after infection at the erythrocyte stage, but not at the tick stage. Taken together, this study characterized from as an antioxidant molecule that was specifically transcribed at the erythrocyte stage.
过氧化物酶（Prxs）是一类抗氧化酶，在硫氧还蛋白、硫氧还蛋白还原酶和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）存在的情况下还原过氧化物，以抵抗氧化应激。在本研究中，我们从[具体来源]中鉴定并分离出一种名为BmTPx-2的2-半胱氨酸过氧化物酶，其全长cDNA为826 bp，开放阅读框为756 bp，编码一个251个氨基酸的蛋白质。BLAST分析表明，BmTPx-2具有2-半胱氨酸过氧化物酶家族成员的典型特征，包括含有两个带有半胱氨酸和保守半胱氨酸残基的保守VCP基序。重组BmTPx-2在[具体表达系统]中表达，并通过蛋白质免疫印迹法进行分析。使用混合功能氧化系统和NADPH氧化证明了BmTPx-2的抗氧化活性。此外，通过实时荧光定量PCR分析了寄生虫在红细胞期和蜱期的BmTPx-2 mRNA表达水平。在红细胞期感染后8天检测到BmTPx-2 mRNA转录峰值，但在蜱期未检测到。综上所述，本研究将来自[具体来源]的BmTPx-2鉴定为一种在红细胞期特异性转录的抗氧化分子。
