Maser Raelene E, Lenhard M James, Pohlig Ryan T, Balagopal P Babu
Department of Medical Laboratory Sciences, University of Delaware, Newark, DE 19716, USA.
Diabetes and Metabolic Research Center, Christiana Care Health System, Newark, DE 19713, USA.
J Clin Transl Endocrinol. 2016 Dec 10;7:7-11. doi: 10.1016/j.jcte.2016.12.002. eCollection 2017 Mar.
Lipoprotein lipase (LPL) is a major enzyme in lipid metabolism. Dyslipidemia, characterized by decreased high-density lipoprotein cholesterol (HDL-C), is prevalent in persons with type 2 diabetes mellitus (T2DM). The aim of this study was to determine whether pre-heparin LPL mass mediates the association between adiponectin and HDL-C in individuals with T2DM.
Pre-heparin LPL mass was measured via an enzyme-linked immunosorbent assay, adiponectin by radioimmunoassay, and HDL-C was determined enzymatically. Participants' (n = 50) demographics, HbA1c, adiposity, homeostasis model assessment for insulin resistance (HOMA-IR), serum creatinine, and lipids were measured. Path analysis was utilized to test whether pre-heparin LPL mass is a mediator in the relationship between adiponectin and HDL-C.
All four criteria for mediation were satisfied in the path analysis. The indirect effect of adiponectin on HDL-C through pre-heparin LPL mass was significant, p = 0.001, whereas the direct effect of adiponectin on HDL-C was not significant, p = 0.074. These results remained consistent even after adjustments for age, gender, body mass index, HOMA-IR, and serum creatinine in the model.
The findings in this study suggest that pre-heparin LPL mass may mediate the association between adiponectin and HDL-C in T2DM. This relationship for measures of HDL-C functionality requires future investigation.
脂蛋白脂肪酶（LPL）是脂质代谢中的一种主要酶。以高密度脂蛋白胆固醇（HDL-C）降低为特征的血脂异常在2型糖尿病（T2DM）患者中很普遍。本研究的目的是确定在T2DM个体中，肝素前LPL质量是否介导脂联素与HDL-C之间的关联。
通过酶联免疫吸附测定法测量肝素前LPL质量，通过放射免疫测定法测量脂联素，并通过酶法测定HDL-C。测量参与者（n = 50）的人口统计学特征、糖化血红蛋白、肥胖程度、胰岛素抵抗的稳态模型评估（HOMA-IR）、血清肌酐和血脂。采用路径分析来检验肝素前LPL质量是否是脂联素与HDL-C之间关系的中介因素。
路径分析满足了所有四个中介标准。脂联素通过肝素前LPL质量对HDL-C的间接效应显著，p = 0.001，而脂联素对HDL-C的直接效应不显著，p = 0.074。即使在模型中对年龄、性别、体重指数、HOMA-IR和血清肌酐进行调整后，这些结果仍然一致。
本研究结果表明，肝素前LPL质量可能介导T2DM中脂联素与HDL-C之间的关联。HDL-C功能指标的这种关系需要未来进一步研究。
