Du Guang-Yu, He Sheng-Wei, Sun Chuan-Xiu, Mi Li-Dong
Department of Bone surgery, The Second Affiliated Hospital of Dalian Medical University, No.216, Shanzhong Street, Ganjing District, Dalian, 116031, Liaoning, People's Republic of China.
Nanoscale Res Lett. 2017 Oct 25;12(1):573. doi: 10.1186/s11671-017-2316-1.
There is an increasing demand for formulations of silk fibroin (SF) scaffolds in biomedical applications. SF was crosslinked via glutaraldehyde with osteoinductive recombinant human bone morphogenic protein-2 (rhBMP2) of different ratios viz. (i) 3% SF with no rhBMP2 (SF), (ii) 3% SF with equal amount of rhBMP2 (SF+BMP2), and (iii) 12% SF with 3% of rhBMP2 (4SF+BMP2), and these solutions were used in electrospinning-based fabrication of nanoscaffolds for evaluating increased osteoinductive potential of SF scaffolds with rhBMP2. Stress-strain relationship suggested there is no loss in mechanical strength of fibers with addition of rhBMP2, and mechanical strength of scaffold was improved with increase in concentration of SF. rhBMP2 association increased the water retention capacity of scaffold as evident from swelling studies. Viability of hMSCs was found to be higher in conjugated scaffolds, and scaffolds do not exhibit any cytotoxicity towards guest cells. Cells were found to have higher alkaline phosphatase activity in conjugated scaffolds under in vitro and in vivo conditions which establishes the increased osteoinductivity of the novel construct. The scaffolds were found to be effective for in vivo bone formation as well.
生物医学应用中对丝素蛋白（SF）支架制剂的需求日益增加。通过戊二醛将SF与不同比例的骨诱导性重组人骨形态发生蛋白-2（rhBMP2）交联，即：（i）不含rhBMP2的3% SF（SF），（ii）等量rhBMP2的3% SF（SF+BMP2），以及（iii）含3% rhBMP2的12% SF（4SF+BMP2），这些溶液用于基于静电纺丝制备纳米支架，以评估含rhBMP2的SF支架增加的骨诱导潜力。应力-应变关系表明，添加rhBMP2后纤维的机械强度没有损失，并且支架的机械强度随着SF浓度的增加而提高。从肿胀研究可以明显看出，rhBMP2的结合增加了支架的保水能力。发现hMSC在共轭支架中的活力更高，并且支架对宿主细胞没有任何细胞毒性。发现在体外和体内条件下，细胞在共轭支架中具有更高的碱性磷酸酶活性，这证实了新型构建体增加的骨诱导性。还发现这些支架对体内骨形成也有效。
