Srivastava Niraj Kumar
School of Life Sciences (SLS), Jawaharlal Nehru University (JNU), JNU Ring Rd, New Delhi, 110067, USA.
Methods Mol Biol. 2018;1687:195-204. doi: 10.1007/978-1-4939-7374-3_14.
Proton nuclear magnetic resonance spectroscopy (H NMR) is a useful tool for the analysis of lipid components in biofluids such as serum/plasma. Such tool is applied for the analysis of lipid components in serum/plasma of patients with Duchenne muscular dystrophy (DMD). The practical approach of sampling, storage, lipid extraction procedure, sample preparation before performing the H NMR spectroscopy experiments is presented. All the experimental parameters of NMR spectroscopy are also described. Details of the assignments of lipid components (qualitative analysis) and quantification of particular lipid components (quantitative analysis) are explained.
质子核磁共振波谱法（¹H NMR）是分析血清/血浆等生物流体中脂质成分的有用工具。该工具用于分析杜氏肌营养不良症（DMD）患者血清/血浆中的脂质成分。本文介绍了采样、储存、脂质提取程序以及在进行¹H NMR波谱实验之前的样品制备的实际方法。还描述了核磁共振波谱的所有实验参数。解释了脂质成分的归属细节（定性分析）和特定脂质成分的定量（定量分析）。
