Tian Zhengshan, Wang Zechun, Han Xinxin, Wang Ning, Wang Ruiyong
School of Chemistry and Environmental Engineering, Pingdingshan University, Pingdingshan, China.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
J Mol Recognit. 2018 Feb;31(2). doi: 10.1002/jmr.2682. Epub 2017 Oct 25.
The interaction between cannabinol (CBN) and herring-sperm deoxyribonucleic acid was investigated by using acridine orange as a fluorescence probe in this work. UV-Vis spectroscopy, fluorescence spectroscopy, and DNA melting techniques were used. The fluorescence of DNA acridine orange was quenched by CBN. The results indicated that CBN can bind to DNA. The binding constant for the CBN and herring-sperm deoxyribonucleic acid was obtained at 3 temperatures, respectively. Results of molecular docking corroborated the experimental results obtained from spectroscopic investigations. The influence of ionic strength on the fluorescence properties was also investigated. The thermodynamic results indicated that hydrophobic interaction played a major role in the binding between CBN and DNA.
在这项工作中，使用吖啶橙作为荧光探针研究了大麻酚（CBN）与鲱鱼精脱氧核糖核酸之间的相互作用。采用了紫外-可见光谱、荧光光谱和DNA熔解技术。CBN使吖啶橙-DNA的荧光猝灭。结果表明CBN能与DNA结合。分别在3个温度下获得了CBN与鲱鱼精脱氧核糖核酸的结合常数。分子对接结果证实了光谱研究获得的实验结果。还研究了离子强度对荧光性质的影响。热力学结果表明，疏水相互作用在CBN与DNA的结合中起主要作用。
