使用各种人类α链和β链cDNA探针观察到的马II类主要组织相容性复合体基因的限制性片段长度多态性。
Restriction fragment length polymorphisms of horse class II MHC genes observed using various human alpha- and beta-chain cDNA probes.
作者信息
Hänni K, Hesford F, Lazary S, Gerber H
机构信息
Klinik fur Nutztiere und Pferde, Institute of Animal Husbandry, University of Berne, Switzerland.
出版信息
Anim Genet. 1988;19(4):395-408. doi: 10.1111/j.1365-2052.1988.tb00831.x.
Genomic DNA isolated from 20 horses was digested with up to six restriction endonucleases and subjected to southern blot hybridization analysis using various human class II alpha- and beta-chain cDNA probes. A high degree of restriction fragment length polymorphism (RFLP) was found for the DQ alpha, DP beta, DQ beta and DR beta probes, about 20 polymorphic bands being detected for each. DR alpha showed 2-4 polymorphic bands, whereas no evidence for DP alpha-like genes was found. A number of correlations of RFLPs with individual alloantisera were apparent.
从20匹马中分离出的基因组DNA用多达六种限制性内切酶进行消化，并用各种人类II类α链和β链cDNA探针进行Southern印迹杂交分析。发现DQα、DPβ、DQβ和DRβ探针存在高度的限制性片段长度多态性（RFLP），每种探针大约检测到20条多态性条带。DRα显示出2 - 4条多态性条带，而未发现DPα样基因的证据。RFLP与个体同种抗血清之间存在许多明显的相关性。
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