应用水解探针分析法鉴定来自坦桑尼亚东北部穆赫扎的严格意义上的疟疾媒介蚊虫——斯氏按蚊的进化枝类型。

Application of hydrolysis probe analysis to identify clade types of the malaria vector mosquito Anopheles funestus sensu stricto from Muheza, northeastern Tanzania.

作者信息

Kweka E J, Mausa E A, Venter N, Derua Y A, Kimaro E E, Coetzee M

机构信息

Division of Livestock and Human Diseases Vector Control, Tropical Pesticides Research Institute, Arusha, Tanzania.

Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, Mwanza, Tanzania.

出版信息

Med Vet Entomol. 2018 Mar;32(1):125-128. doi: 10.1111/mve.12274. Epub 2017 Oct 25.

DOI:10.1111/mve.12274

PMID:29068089

Abstract

A hydrolysis probe analysis (TaqMan assay) was used to study clade types in Anopheles funestus sensu stricto Giles, a major malaria vector in sub-Saharan Africa, with specimens collected from Muheza in Tanga, northeastern Tanzania. A total of 186 An. funestus specimens were analysed, revealing that 176 (94.6%) were of clade I and 10 (5.4%) of clade II. These findings extend the distribution of clade type II from southern Mozambique and northern Zambia to northeastern Tanzania. The technique used can also be of great value in assessing the role and contribution of these clade types in malaria transmission and insecticide resistance frequencies for An. funestus s.s.

摘要

采用水解探针分析（TaqMan 检测法）对非洲撒哈拉以南地区主要疟疾传播媒介——严格意义上的冈比亚按蚊（Anopheles funestus sensu stricto Giles）的进化枝类型进行研究，样本采集自坦桑尼亚东北部坦噶的穆赫扎。共分析了 186 只冈比亚按蚊样本，结果显示，176 只（94.6%）属于进化枝 I，10 只（5.4%）属于进化枝 II。这些发现将进化枝 II 的分布范围从莫桑比克南部和赞比亚北部扩展至坦桑尼亚东北部。所采用的技术在评估这些进化枝类型在疟疾传播中的作用和贡献以及冈比亚按蚊的杀虫剂抗性频率方面也具有重要价值。