Tungu Patrick K, Waweru John, Karthi Sengodan, Wangai James, Kweka Eliningaya J
National Institute for Medical Research, Amani Medical Research Centre, P.O. Box 81, Muheza Tanga, Tanzania.
Department of Disease Control, London School of Hygiene and Tropical Medicine, London, UK.
Curr Res Parasitol Vector Borne Dis. 2021 May 31;1:100030. doi: 10.1016/j.crpvbd.2021.100030. eCollection 2021.
The success of long-lasting insecticidal nets (LLIN) as the primary method for preventing malaria is threatened by pyrethroid resistance in vectors. New generation long-lasting nets incorporating PBO synergist (piperonyl butoxide) with pyrethroid are designed to control insecticide-resistant mosquitoes. The efficacy of Veeralin® PBO LLINs was evaluated in experimental huts against wild free-flying pyrethroid-resistant (). Mosquito mortality, blood-feeding inhibition and personal protection were compared between untreated nets, standard LLINs and PBO/pyrethroid combination nets. Blood-feeding rates recorded with 20-times washed Veeralin were not significantly different from those with 20-times washed PermaNet 3.0 LLIN, a WHO Pre-Qualification Team (PQT) approved PBO/pyrethroid LLIN. This provides evidence that Veeralin LLIN provides similar blood-feeding inhibition to the standard approved LLIN and thus meets WHO PQT criteria for blood-feeding. Results show significantly higher mortality for Veeralin PBO LLINs against pyrethroid-resistant () compared to DuraNet, a WHO PQT approved standard pyrethroid-only LLIN, both when unwashed and washed 20 times. The improved efficacy over a standard pyrethroid-only LLIN can be attributed to the effect of PBO in the Veeralin LLIN, hence meeting the Vector Control Advisory Group (VCAG) criteria for a resistance breaking LLIN.
长效杀虫网（LLIN）作为预防疟疾的主要方法，其成功正受到病媒对拟除虫菊酯产生抗性的威胁。新一代将增效醚（胡椒基丁醚）与拟除虫菊酯结合的长效蚊帐旨在控制对杀虫剂具有抗性的蚊子。在实验小屋中评估了Veeralin® PBO LLINs对野生自由飞行的拟除虫菊酯抗性（）的效果。比较了未处理蚊帐、标准LLINs和PBO/拟除虫菊酯组合蚊帐之间的蚊子死亡率、取食抑制和个人防护情况。用洗涤20次后的Veeralin记录的取食率与经世界卫生组织预认证小组（PQT）批准的PBO/拟除虫菊酯LLIN——PermaNet 3.0 LLIN洗涤20次后的取食率没有显著差异。这提供了证据，表明Veeralin LLIN对取食的抑制作用与标准批准的LLIN相似，因此符合世界卫生组织PQT关于取食抑制的标准。结果表明，与世界卫生组织PQT批准的仅含拟除虫菊酯的标准LLIN——DuraNet相比，无论未洗涤还是洗涤20次，Veeralin PBO LLINs对拟除虫菊酯抗性（）的蚊子的死亡率都显著更高。与仅含拟除虫菊酯的标准LLIN相比，其效果的改善可归因于Veeralin LLIN中增效醚的作用，因此符合病媒控制咨询小组（VCAG）关于抗药性突破LLIN的标准。
