Pindzola R R, Ho R H, Martin G F
Department of Anatomy, Ohio State University College of Medicine, Columbus.
Brain Behav Evol. 1988;32(5):281-92. doi: 10.1159/000116557.
Axons presumed to contain catecholamines were visualized in the spinal cord of the spinal cord of the North American opossum using antibodies to tyrosine hydroxylase (TH) by an indirect antibody peroxidase-antiperoxidase (PAP) technique. Axons showing TH-like immunoreactivity (TH-IR) coursed primarily in the dorsal part of the lateral funiculus, although they were also present more ventrally. An occasional TH-IR axon was seen in the dorsal funiculus. Within the gray matter, TH-IR axons were distributed most densely within the intermediolateral cell column (ILC). Such axons were more numerous in the presumptive sacral parasympathetic nucleus than within the adjacent gray matter, but their density was less than that within the ILC. Many TH-IR axons were also found within laminae I-V and X, but the density of innervation in the ventral horn was relatively low. The brainstem origin of TH-IR axons in the spinal cord was studied using a combination of the retrograde transport of fluorescent dyes and immunofluorescence as well as the retrograde transport of horseradish peroxidase and PAP immunohistochemistry. The injections of retrograde markers were made at either cervical, thoracic or lumbar spinal levels. With both techniques, spinally projecting TH-IR neurons were located within the nucleus periventricularis hypothalami, the nucleus paraventricularis hypothalami dorsalis, the area hypothalamica posterior, the ventral part of the nucleus coeruleus, the nucleus coeruleus pars alpha, the lateral part of the nucleus reticularis pontis, and several nuclei of the ventrolateral medulla. Few or no cells within the parabrachial area, the region of the Kölliker-Fuse nucleus or the area adjacent to the superior olivary complex (the location of the A5 group of rats) provided TH-IR projections to the spinal cord. Our results suggest that catecholaminergic projections to the spinal cord of the marsupial opossum are similar in termination and origin to those described for rats and other placental mammals, but differences do exist.
利用抗酪氨酸羟化酶（TH）抗体，通过间接抗体过氧化物酶 - 抗过氧化物酶（PAP）技术，在北美负鼠的脊髓中观察到了推测含有儿茶酚胺的轴突。显示TH样免疫反应性（TH-IR）的轴突主要在外侧索的背侧走行，不过在更腹侧也有。偶尔在背侧索可见TH-IR轴突。在灰质内，TH-IR轴突在中间外侧细胞柱（ILC）内分布最密集。在推测的骶副交感神经核中，这类轴突比相邻灰质中的更多，但其密度低于ILC内的密度。在I - V层和X层内也发现了许多TH-IR轴突，但腹角的神经支配密度相对较低。使用荧光染料逆行运输和免疫荧光相结合的方法，以及辣根过氧化物酶逆行运输和PAP免疫组织化学方法，研究了脊髓中TH-IR轴突的脑干起源。逆行标记物注射在颈、胸或腰脊髓水平。两种技术都显示，投射到脊髓的TH-IR神经元位于室周下丘脑核、下丘脑背侧室旁核、下丘脑后区、蓝斑核腹侧部、蓝斑核α部、脑桥网状核外侧部以及延髓腹外侧的几个核内。臂旁区、柯利克 - 富斯核区域或与上橄榄复合体相邻区域（大鼠A5组的位置）内很少或没有细胞向脊髓提供TH-IR投射。我们的结果表明，有袋类负鼠脊髓的儿茶酚胺能投射在终末和起源方面与大鼠及其他胎盘哺乳动物所描述的相似，但确实存在差异。
