Department of Pulmonary Oncology, Affiliated Hospital of the Academy of Military Medical Sciences, Beijing, China.
Department of Immunology, Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Thorac Cancer. 2018 Jan;9(1):44-50. doi: 10.1111/1759-7714.12526. Epub 2017 Oct 25.
This study was conducted to evaluate the clinical utility of the oHSV1-hTERT-GFP circulating tumor cell (CTC) detection method in the peripheral blood of patients with lung cancer by comparing its sensitivity to the CellSearch CTC detection method.
The oHSV1-hTERT-GFP and CellSearch CTC detection methods were compared using peripheral blood samples of patients pathologically diagnosed with lung cancer.
A total of 240 patients with lung cancer were recruited, including 89 patients who were newly diagnosed and 151 patients who had previously received treatment. Sixty-six newly diagnosed patients were evaluated using both methods. The CTC detection rates were 71.2% and 33.3% using the oHSV1-hTERT-GFP and CellSearch methods, respectively; this difference was statistically significant (P = 0.000). Among the entire cohort (n = 240), the CTC detection rate using the oHSV1-hTERT-GFP method was 76.3%, with a CTC count of 0-81. The CTC detection rates were 76.7%, 68.9%, and 76.3% in patients with squamous cell carcinoma, adenocarcinoma, and small cell lung cancer, respectively. There was no statistically significant difference in the CTC detection rates between these different pathological subtypes (P = 0.738). The CTC detection rates of 79.8% and 74.4% in patients with stage I-III and IV lung cancer, respectively, were not significantly different (P = 0.427).
The oHSV1-hTERT-GFP method is highly effective for detecting CTCs in patients with lung cancer, independent of pathological type and disease stage, and is ideal for large-scale clinical applications.
本研究旨在通过比较 oHSV1-hTERT-GFP 循环肿瘤细胞（CTC）检测方法与 CellSearch CTC 检测方法的灵敏度，评估其在肺癌患者外周血中的临床应用价值。
采用 oHSV1-hTERT-GFP 和 CellSearch CTC 检测方法对经病理诊断为肺癌的患者的外周血样本进行比较。
共纳入 240 例肺癌患者，包括新诊断患者 89 例和既往治疗患者 151 例。对 66 例新诊断患者同时采用两种方法进行评估。oHSV1-hTERT-GFP 和 CellSearch 方法的 CTC 检出率分别为 71.2%和 33.3%，差异具有统计学意义（P=0.000）。在整个队列（n=240）中，oHSV1-hTERT-GFP 方法的 CTC 检出率为 76.3%，CTC 数为 0-81。鳞癌、腺癌和小细胞肺癌患者的 CTC 检出率分别为 76.7%、68.9%和 76.3%，不同病理亚型之间 CTC 检出率差异无统计学意义（P=0.738）。Ⅰ-Ⅲ期和Ⅳ期肺癌患者的 CTC 检出率分别为 79.8%和 74.4%，差异无统计学意义（P=0.427）。
oHSV1-hTERT-GFP 方法在检测肺癌患者 CTC 方面具有较高的效率，不受病理类型和疾病阶段的影响，是一种理想的用于大规模临床应用的方法。
