Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, SFR143, Federative Institute of Research in Sciences and Health Engineering, Faculty of Medicine, Jean Monnet University, Saint-Etienne, France.
Laboratoires Théa, Clermont-Ferrand, France.
Acta Ophthalmol. 2018 Mar;96(2):e140-e146. doi: 10.1111/aos.13559. Epub 2017 Oct 25.
To describe and validate the micro-instillation of fluorescein on the ocular surface by a disposable calibrated inoculation loop to improve corneal and conjunctival staining quality.
Accuracy and precision of the volume of 0.5% sodium fluorescein collected by a single use 1 μl-calibrated inoculation loop were measured using a precision balance. Twenty patients (40 eyes) suffering from dry eye syndrome were enrolled in a prospective interventional nonrandomized study. Fluorescein was instilled with the loop, and slit-lamp images were taken within 30 seconds using cobalt blue light with and without a yellow barrier filter. For comparison, after a washout period, the same images were retaken after instillation of one drop of fluorescein from a single-dose unit. The main outcome measure was the staining quality assessed by three experts, blind to the instillation method. Patient discomfort (tolerance, by a questionnaire) was also compared.
The mean volume collected by the loop was 1.18 ± 0.12 μl, compared with 33.70 ± 6.10 μl using the single-dose unit. The loop avoided excess dye responsible for unpleasant tearing, masking of lesions and rapid diffusion into the stroma. Micro-instillation greatly improved image quality without losing information. The yellow filter further improved image contrast. Tolerance was excellent.
The 1 μl-calibrated inoculation loop is a safe, convenient, inexpensive, disposable, sterile, well-tolerated tool for reproducible micro-instillation of commercial fluorescein. By greatly improving staining quality, it will help standardize assessment of dry eye severity.
描述并验证通过一次性校准接种环在眼表面微量滴注荧光素，以提高角膜和结膜染色质量。
使用精密天平测量单个 1μl 校准接种环收集的 0.5%荧光素钠的体积的准确性和精密度。20 例（40 只眼）患有干燥综合征的患者纳入前瞻性非随机干预研究。用环滴注荧光素，在 30 秒内使用钴蓝光并带有或不带有黄色滤光片拍摄裂隙灯图像。为了比较，在冲洗期后，在用单剂量单位滴注一滴荧光素后，再次拍摄相同的图像。主要观察指标是三位专家评估的染色质量，评估时对滴注方法不了解。还比较了患者的不适（通过问卷评估的耐受性）。
环收集的平均体积为 1.18±0.12μl，而单剂量单位的体积为 33.70±6.10μl。环避免了过多的染料，这些染料会导致不愉快的流泪、病变的掩盖和快速扩散到基质中。微量滴注极大地提高了图像质量，而不会丢失信息。黄色滤光片进一步提高了图像对比度。耐受性极好。
1μl 校准接种环是一种安全、方便、经济、一次性、无菌、耐受性好的工具，可用于商业荧光素的重复微量滴注。通过极大地提高染色质量，它将有助于标准化干燥性眼严重程度的评估。
