精索静脉曲张男性的精液参数：DNA 片段化、染色质包装、线粒体膜电位和细胞凋亡。
Semen parameters in men with varicocele: DNA fragmentation, chromatin packaging, mitochondrial membrane potential, and apoptosis.
作者信息
Dieamant Felipe, Petersen Claudia G, Mauri Ana L, Conmar Vanessa, Mattila Mariana, Vagnini Laura D, Renzi Adriana, Costa Bruna P, Zamara Camila, Oliveira João Batista A, Baruffi Ricardo L R, Franco José G
机构信息
Center for Human Reproduction Prof Franco Jr. - Ribeirão Preto, Brazil.
Paulista Center for Diagnosis, Research and Training - Ribeirão Preto, Brazil.
出版信息
JBRA Assist Reprod. 2017 Dec 1;21(4):295-301. doi: 10.5935/1518-0557.20170053.
OBJECTIVE
To evaluate varicocele effects on conventional semen parameters: sperm DNA fragmentation, chromatin packaging, mitochondrial membrane potential (MMP) and sperm apoptosis.
METHODS
A cross-sectional study was carried out with semen samples from 2,399 men of couples who attended an infertility clinic. A total of 16.3% (391/2399) of the men were diagnosed with varicocele by a urologist.
RESULTS
A regression analysis revealed that the percentages of sperm with DNA fragmentation, abnormal chromatin packaging, and abnormal MMP were significantly increased in individuals with varicocele, when compared to men without varicocele. Apoptosis was not influenced by varicocele. Conventional semen parameters were significantly worse in individuals with the disease. On the other hand, in men with varicocele, Spearman's correlation demonstrated that early apoptosis and abnormal MMP showed a positive and significant correlation with sperm DNA fragmentation.
CONCLUSION
Men with varicocele had worse semen parameters, including increased levels of sperm DNA fragmentation, inactive mitochondria, and abnormal chromatin packaging. These changes are possible causes of infertility in individuals with varicocele.
目的
评估精索静脉曲张对传统精液参数的影响：精子DNA碎片化、染色质包装、线粒体膜电位（MMP）和精子凋亡。
方法
对前往不孕不育诊所就诊的夫妇中2399名男性的精液样本进行了一项横断面研究。共有16.3%（391/2399）的男性被泌尿科医生诊断为精索静脉曲张。
结果
回归分析显示，与无精索静脉曲张的男性相比，精索静脉曲张患者中DNA碎片化、染色质包装异常和MMP异常的精子百分比显著增加。凋亡不受精索静脉曲张的影响。患有该疾病的个体的传统精液参数明显更差。另一方面，在精索静脉曲张男性中，Spearman相关性分析表明早期凋亡和异常MMP与精子DNA碎片化呈显著正相关。
结论
精索静脉曲张男性的精液参数更差，包括精子DNA碎片化水平升高、线粒体无活性和染色质包装异常。这些变化可能是精索静脉曲张患者不孕的原因。
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