a Department of Biotechnology , Panjab University , Chandigarh , India.
b Department of Cardiology , Postgraduate Institute of Medical Education and Research , Chandigarh , India.
Clin Exp Hypertens. 2018;40(4):398-405. doi: 10.1080/10641963.2017.1384485. Epub 2017 Oct 25.
Coronary artery disease (CAD) is major cause of death and morbidity worldwide. Arachidonate 12/15-lipoxygenase (ALOX) is a member of the lipid peroxidizing enzyme family and implicated in the pathogenesis of atherosclerosis, but with contradicting results.
The present study aimed to investigate the association of two polymorphisms in ALOX15 (rs2619112 and rs7217186) and the risk of CAD in North Indian population.
A total of 500 angiographically confirmed CAD patients and 500 control subjects of North Indian population were recruited in the case-control study and genotyped by PCR-RFLP.
The data showed a significant association between the two polymorphisms and CAD. Multiple logistic regression revealed heterozygous genotype of both the polymorphisms viz. GA of rs2619112 and CT of rs7217186 to be associated with significant high risk of CAD after adjustment for confounders (p = 0.034, OR = 2.274, 95% CI (1.062-4.870) and p = 0.000, OR = 3.407, 95% CI (2.092-5.548) respectively). Stratified analysis based on gender showed GA and AA of rs2619112 each significantly increased the risk of CAD in females (p = 0.001, OR = 13.120, CI = 2.780-61.928; p = 0.028, OR = 5.393, CI = 1.196-24.316 respectively) whereas only GA increased CAD risk in males (p = 0.005, OR = 2.277, CI = 1.290-4.020). In case of rs7217186, CT and TT showed a significant high risk of CAD in males (p = 0.000, OR = 4.048, CI = 2.678-6.119; p = 0.000, OR = 2.861, CI = 1.928-4.245).
The present study shows that rs7217186:C > T and rs2619112:G > A of ALOX15 are associated with increased risk of CAD in the North Indian population.
冠心病（CAD）是全世界死亡和发病的主要原因。花生四烯酸 12/15-脂氧合酶（ALOX）是脂质过氧化酶家族的成员，与动脉粥样硬化的发病机制有关，但结果存在矛盾。
本研究旨在探讨 2 个 ALOX15 多态性（rs2619112 和 rs7217186）与北印度人群 CAD 风险的关系。
采用病例对照研究，共纳入 500 例经血管造影证实的 CAD 患者和 500 例北印度人群对照，采用 PCR-RFLP 法进行基因分型。
数据显示，这两种多态性与 CAD 显著相关。多因素 logistic 回归显示，杂合基因型 GA 均 rs2619112 和 CT rs7217186 与校正混杂因素后 CAD 的显著高风险相关（p=0.034，OR=2.274，95%CI（1.062-4.870）和 p=0.000，OR=3.407，95%CI（2.092-5.548）。基于性别分层分析显示，rs2619112 的 GA 和 AA 分别显著增加了女性 CAD 的风险（p=0.001，OR=13.120，CI=2.780-61.928；p=0.028，OR=5.393，CI=1.196-24.316），而仅 GA 增加了男性 CAD 的风险（p=0.005，OR=2.277，CI=1.290-4.020）。对于 rs7217186，CT 和 TT 显示男性 CAD 的高风险（p=0.000，OR=4.048，CI=2.678-6.119；p=0.000，OR=2.861，CI=1.928-4.245）。
本研究表明，ALOX15 的 rs7217186：C>T 和 rs2619112：G>A 与北印度人群 CAD 风险增加相关。
