Metineren Hasan, Dülgeroğlu Turan Cihan, Metineren Mehmet Hüseyin, Aydın Ekrem
Department of Orthopedics and Traumatology, Medical Faculty, Dumlupınar University, Turkey.
Department of Pathology, Medical Faculty, Dumlupınar University, Turkey.
Adv Clin Exp Med. 2017 Sep;26(6):919-923. doi: 10.17219/acem/66291.
Bone metabolism is a complex system, and fracture healing is one of its most important functions. Many circumstances can influence this process. Chronic drug use in elderly populations can affect bone healing, and inadequate tissue perfusion, increased free radicals and adverse drug effects can negatively influence fracture healing. Nebivolol, an anti-hypertensive drug that selectively blocks β1 receptors, effectively reduces blood pressure by inducing peripheral vasodilation. Nebivolol also exerts anti-oxidant effects by stimulating nitric oxide (NO) synthesis. Many studies show that NO protects the vascular endothelium and improves fracture healing.
In this study, the histological and radiological effects of intraperitoneally administered nebivolol on fracture healing were evaluated.
Twenty-one Sprague Dawley rats were divided into 3 (nebivolol 1, 2 and control) groups. Sterile nebivolol solution (1 mL = 0.017 mg nebivolol) was given to the rats in group 1 every day for 4 weeks, while the rats in nebivolol group 2 were given 2 mL per day, beginning after the production of an open, displaced unilateral femur fracture. Radiographic and histological studies were used to evaluate fracture healing.
Histological and immunohistochemical analysis showed osseous healing with woven bone at the fracture site and only minimal amounts of cartilage in nebivolol 1 and 2 groups. Radiological grading was not different between the control and the nebivolol groups.
This study suggests that nebivolol, a selective β blocker, has positive effects on fracture healing through anti-oxidative effects via the NO pathway and direct vasodilator effects.
骨代谢是一个复杂的系统，骨折愈合是其最重要的功能之一。许多情况会影响这一过程。老年人群长期用药会影响骨愈合，组织灌注不足、自由基增加和药物不良反应会对骨折愈合产生负面影响。奈必洛尔是一种选择性阻断β1受体的抗高血压药物，通过诱导外周血管舒张有效降低血压。奈必洛尔还通过刺激一氧化氮（NO）合成发挥抗氧化作用。许多研究表明，NO可保护血管内皮并促进骨折愈合。
本研究评估腹腔注射奈必洛尔对骨折愈合的组织学和放射学影响。
将21只Sprague Dawley大鼠分为3组（奈必洛尔1组、2组和对照组）。第1组大鼠每天给予无菌奈必洛尔溶液（1 mL = 0.017 mg奈必洛尔），持续4周，而奈必洛尔2组大鼠在造成开放性、移位性单侧股骨骨折后每天给予2 mL。采用放射学和组织学研究评估骨折愈合情况。
组织学和免疫组织化学分析显示，奈必洛尔1组和2组骨折部位有编织骨骨愈合，软骨量极少。对照组和奈必洛尔组之间的放射学分级无差异。
本研究表明，选择性β受体阻滞剂奈必洛尔通过NO途径的抗氧化作用和直接血管舒张作用对骨折愈合有积极影响。
