Liu Pengcheng, Liu Junfeng, Xia Kuo, Chen Liyang, Wu Xing
Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, P.R. China.
Sci Rep. 2016 Jul 29;6:30754. doi: 10.1038/srep30754.
To evaluate the effect of leptin combined with CoCl2 on rat femur fracture healing. 48 male Sprague Dawley rats were randomly divided into two main groups. Then standardized femur fractures were created to all rats. Control group rats were treated with 0.5 mL physiological saline, and experimental group rats were treated with 5 μg/Kg.d leptin and 15 mg/Kg.d CoCl2 along with 0.5 mL physiological saline for 42 days intraperitoneally. Each main group was divided into three subgroups for each evaluation at second, fourth and sixth weeks, each subgroup included eight rats. The radiological evaluation showed that the fracture healing progress of experimental group was superior to control group from second week. At fourth week, experimental group had better fracture healing progress than control group significantly. Results of biomechanics show the ultimate load (N) and deflection ultimate load (mm) of experimental group was significantly increased than that in control group from fourth week. The present result demonstrated that leptin combined with CoCl2 significantly increased the mRNA expression levels of HIF1A, Vegfa, Runx2, Bmp2, Bglap and Alpl. It suggested that leptin combined with CoCl2 have a positive effect on rat femur fracture healing by activating the HIF1A pathway.
为评估瘦素联合氯化钴对大鼠股骨骨折愈合的影响。将48只雄性Sprague Dawley大鼠随机分为两个主要组。然后对所有大鼠制造标准化的股骨骨折。对照组大鼠腹腔注射0.5 mL生理盐水，实验组大鼠腹腔注射5 μg/Kg.d瘦素、15 mg/Kg.d氯化钴及0.5 mL生理盐水，持续42天。每个主要组在第二、第四和第六周各分为三个亚组进行每次评估，每个亚组包括8只大鼠。影像学评估显示，从第二周起实验组的骨折愈合进程优于对照组。在第四周时，实验组的骨折愈合进程明显优于对照组。生物力学结果显示，从第四周起实验组的极限载荷（N）和挠曲极限载荷（mm）均显著高于对照组。目前的结果表明，瘦素联合氯化钴显著提高了HIF1A、Vegfa、Runx2、Bmp2、Bglap和Alpl的mRNA表达水平。提示瘦素联合氯化钴通过激活HIF1A通路对大鼠股骨骨折愈合有积极作用。
