College of Chemistry, Jilin University , Changchun 130012, China.
China-Japan Hospital of Jilin University , Changchun 130033, China.
Biomacromolecules. 2017 Dec 11;18(12):3971-3977. doi: 10.1021/acs.biomac.7b01003. Epub 2017 Nov 7.
Biological-material-functionalized porous monoliths were prepared with lactoferrin and β-cyclodextrin via a click reaction. With the monolith as an extraction medium, a method combined with ICP-MS was developed for the determination of total gallium originating from metabolic residues of orally bioavailable gallium complexes with tris(8-quinolinolato)gallium (GaQ) as a representative. The method exhibited favorable adsorption behaviors for gallium with high selectivity, low detection limit (2 ng L), and an enrichment factor of 29-fold with the sample throughput of 30 min. The developed approach was validated by the analysis of gallium from GaQ metabolic residues in a human cell line. Additionally, the practical applicability of this method was evaluated by the determination of gallium in human blood and urine samples from cancer patients. Results illustrated that the prepared monolith had potential in Ga-based anticancer drug analysis in complex biological samples.
采用点击反应，以乳铁蛋白和β-环糊精为功能化试剂，制备了生物材料功能化多孔整体材料。以该整体材料为萃取介质，结合电感耦合等离子体质谱法，建立了一种测定口服生物利用度镓配合物（以三（8-羟基喹啉）镓（GaQ）为代表）代谢残渣中总镓的方法。该方法对镓表现出良好的吸附行为，具有高选择性、低检测限（2 ng·L-1）和 29 倍的富集因子，样品分析时间为 30 min。通过分析人细胞系中 GaQ 代谢残渣中的镓对该方法进行了验证。此外，还通过测定癌症患者的人血和尿样中的镓来评估该方法的实际适用性。结果表明，该制备的整体材料在复杂生物样品中基于 Ga 的抗癌药物分析方面具有潜在应用价值。
