Zheng Hai-Jiao, Zhao Ya-Ming, Wu Xiao-Feng, Xu Hai, Jia Qiong
College of Chemistry, Jilin University, Changchun 130012, China.
Se Pu. 2025 Mar;43(3):289-294. doi: 10.3724/SP.J.1123.2024.04025.
"Admiring the lofty mountains, one realizes their own lack of talent." To further promote the implementation of new curriculum standards, a comprehensive instrumental analysis experiment was designed. Metabolomics, an emerging technology developed after genomics and proteomics, is an important part of systems biology. This study aims to explore the applications of metabolomics in the fields of environment and health. Sample pretreatment technology and detection methods for separating and enriching gallium were chosen through a literature review and group discussion. Students then experimentally analyzed the changes in metabolite content in cells cultured with metal-anticancer gallium drugs, which helped them understand the widespread application of metabolomics in the fields of environment and health. Additionally, material characterization was conducted using X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TG), and the gallium metal in metabolites was qualitatively and quantitatively analyzed using inductively coupled plasma mass spectrometry (ICP-MS). During discussions of this experiment, the teacher guided students in using large-scale instruments to solve problems comprehensively, fostering a research-based teaching approach to build a solid foundation for conducting efficient instrument analysis and comprehensive experiments within the classroom in future.
“仰山而小己之才。”为进一步推动新课程标准的实施，设计了一项综合仪器分析实验。代谢组学是继基因组学和蛋白质组学之后发展起来的一项新兴技术，是系统生物学的重要组成部分。本研究旨在探讨代谢组学在环境与健康领域的应用。通过文献综述和小组讨论，选择了分离和富集镓的样品预处理技术和检测方法。然后，学生们通过实验分析了用金属抗癌镓药物培养的细胞中代谢物含量的变化，这有助于他们了解代谢组学在环境与健康领域的广泛应用。此外，使用X射线光电子能谱（XPS）和热重分析（TG）进行材料表征，并使用电感耦合等离子体质谱（ICP-MS）对代谢物中的镓金属进行定性和定量分析。在本次实验讨论过程中，教师引导学生使用大型仪器全面解决问题，培养基于研究的教学方法，为今后在课堂内高效开展仪器分析和综合实验奠定坚实基础。
