Zhou Xiao-Yi, Wu Sui-Yi, Zhang Zi-Cheng, Wang Fei, Yang Yi-Lin, Li Ming, Wei Xian-Zhao
Department of Orthopedic Surgery, Changhai Hospital Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
Medicine (Baltimore). 2017 Oct;96(43):e8397. doi: 10.1097/MD.0000000000008397.
Angiogenesis plays an important role during bone regeneration. Low-intensity pulsed ultrasound (LIPUS) has been proven to accelerate the process of bone fracture healing. However, the mechanism of the effect of LIPUS on bone regeneration is still unclear. In the present study, we used human umbilical vein endothelial cell (HUVEC) and human osteosarcoma cell (MG-63) to investigate the effect of LIPUS stimulation in an endothelial cell-osteoblast coculture system. At the same time, we used transwell and in vitro angiogenesis assay to observe how LIPUS affects endothelial cells. The results demonstrated that LIPUS could significantly increase the migratory ability and promote tube formation in angiogenesis of HUVECs. Furthermore, LIPUS could significantly elevate the expression of osteogenesis-related genes on osteoblasts such as Runt-related transcription factor 2, alkaline phosphatase, Osteorix, and Cyclin-D1, indicating the pro-osteogenesis effect of LIPUS in our coculture system. In conclusion, endothelial cell is involved in LIPUS-accelerated bone regeneration, the positive effect of LIPUS may be transferred via endothelial cells surrounding fracture healing site.
血管生成在骨再生过程中发挥着重要作用。低强度脉冲超声（LIPUS）已被证明可加速骨折愈合进程。然而，LIPUS对骨再生作用的机制仍不清楚。在本研究中，我们使用人脐静脉内皮细胞（HUVEC）和人骨肉瘤细胞（MG-63），在一个内皮细胞-成骨细胞共培养体系中研究LIPUS刺激的作用。同时，我们使用Transwell和体外血管生成试验来观察LIPUS如何影响内皮细胞。结果表明，LIPUS可显著提高HUVECs在血管生成中的迁移能力并促进管腔形成。此外，LIPUS可显著提高成骨细胞上成骨相关基因如Runt相关转录因子2、碱性磷酸酶、Osterix和细胞周期蛋白D1的表达，表明在我们的共培养体系中LIPUS具有促进成骨的作用。总之，内皮细胞参与了LIPUS加速的骨再生过程，LIPUS的积极作用可能通过骨折愈合部位周围的内皮细胞传递。
