Department of Prosthodontics, School and Hospital of Stomatology, China Medical University, Shenyang 110002, China.
Liaoning Provincial Key Laboratory of Oral Diseases, China Medical University, Shenyang 110002, China.
Biomolecules. 2024 Sep 13;14(9):1154. doi: 10.3390/biom14091154.
Porous Ti-6Al-4V scaffold materials can be used to heal massive bone defects because they can provide space for vascularisation and bone formation. During new bone tissue development, rapid vascular ingrowth into scaffold materials is very important. Osteoblast-derived exosomes are capable of facilitating angiogenesis-osteogenesis coupling. Low-intensity pulsed ultrasound (LIPUS) is a physical therapy modality widely utilised in the field of bone regeneration and has been proven to enhance the production and functionality of exosomes on two-dimensional surfaces. The impact of LIPUS on exosomes derived from osteoblasts cultured in three dimensions remains to be elucidated. In this study, exosomes produced by osteoblasts on porous Ti-6Al-4V scaffold materials under LIPUS and non-ultrasound stimulated conditions were co-cultured with endothelial cells. The findings indicated that the exosomes were consistently and stably taken up by the endothelial cells. Compared to the non-ultrasound group, the LIPUS group facilitated endothelial cell proliferation and angiogenesis. After 24 h of co-culture, the migration ability of endothelial cells in the LIPUS group was 17.30% higher relative to the non-ultrasound group. LIPUS may represent a potentially viable strategy to promote the efficacy of osteoblast-derived exosomes to enhance the angiogenesis of porous Ti-6Al-4V scaffold materials.
多孔 Ti-6Al-4V 支架材料可用于治疗大段骨缺损，因为它们可以为血管生成和骨形成提供空间。在新骨组织发育过程中，快速血管向支架材料内生长非常重要。成骨细胞来源的外泌体能够促进血管生成与成骨偶联。低强度脉冲超声（LIPUS）是一种在骨再生领域广泛应用的物理治疗方式，已被证明可提高二维表面上外泌体的产生和功能。LIPUS 对三维培养的成骨细胞来源的外泌体的影响仍有待阐明。在这项研究中，将成骨细胞在多孔 Ti-6Al-4V 支架材料上在 LIPUS 和非超声刺激条件下产生的外泌体与内皮细胞共培养。结果表明，内皮细胞始终稳定地摄取外泌体。与非超声组相比，LIPUS 组促进了内皮细胞的增殖和血管生成。共培养 24 h 后，LIPUS 组内皮细胞的迁移能力比非超声组高 17.30%。LIPUS 可能是一种有前途的策略，可以提高成骨细胞来源的外泌体的功效，以增强多孔 Ti-6Al-4V 支架材料的血管生成。
