XdhR负向调控天蓝色链霉菌M145中放线紫红素的生物合成。
XdhR negatively regulates actinorhodin biosynthesis in Streptomyces coelicolor M145.
作者信息
Fu Jiafang, Zong Gongli, Zhang Peipei, Zhao Zhilong, Ma Junxia, Pang Xiuhua, Cao Guangxiang
机构信息
Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan, Jingshi Road 18877, Jinan 250062, Shandong, China.
State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nanlu, Jinan 250100, China.
出版信息
FEMS Microbiol Lett. 2017 Dec 1;364(22). doi: 10.1093/femsle/fnx226.
The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overproduction of actinorhodin by approximately 2.5-fold compared to the wild-type strain. Complementation of the xdhR deletion strain restored actinorhodin production to normal levels. In addition, the relative expression levels of actinorhodin cluster genes were all significantly increased in the xdhR deletion strain compared to the wild-type strain. XdhR can specifically bind the promoters of actII-4 and actII-1, two pathway-specific regulators of actinorhodin biosynthesis. These results suggest that xdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in S. coelicolor.
xdhR基因在天蓝色链霉菌中编码一种TetR家族调控因子。然而，关于XdhR在调节放线紫红素产生方面的功能却知之甚少。在此，我们报道XdhR在天蓝色链霉菌中对放线紫红素生物合成起负调控作用。与野生型菌株相比，xdhR缺失导致放线紫红素过量产生约2.5倍。xdhR缺失菌株的互补使放线紫红素产量恢复到正常水平。此外，与野生型菌株相比，xdhR缺失菌株中放线紫红素簇基因的相对表达水平均显著增加。XdhR可以特异性结合actII-4和actII-1的启动子，这两个是放线紫红素生物合成的途径特异性调控因子。这些结果表明，xdhR通过直接调控天蓝色链霉菌中的actII-4和actII-1对放线紫红素生物合成起负控制作用。
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