Department of Orthodontic Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Oral Dis. 2018 May;24(4):600-610. doi: 10.1111/odi.12798. Epub 2017 Nov 24.
This study evaluated low-intensity pulsed ultrasound effects for temporomandibular joint osteoarthritis in adult rats.
Osteoarthritis-like lesions were induced in 24 adult rats' temporomandibular joints with low-dose mono-iodoacetate injections. The rats were divided into four groups: control and mono-iodoacetate groups, injected with contrast media and mono-iodoacetate, respectively, at 12 weeks and observed until 20 weeks; and low-intensity pulsed ultrasound and mono-iodoacetate + low-intensity pulsed ultrasound groups, injected with contrast media and mono-iodoacetate, respectively, at 12 weeks with low-intensity pulsed ultrasound performed from 16 to 20 weeks. Condylar bone mineral density, bone mineral content and bone volume were evaluated weekly with microcomputed tomography. Histological and immunohistochemical staining for matrix metalloproteinases-13 was performed at 20 weeks.
At 20 weeks, the mono-iodoacetate + low-intensity pulsed ultrasound group showed significantly higher bone mineral density, bone mineral content and bone volume than the mono-iodoacetate group; however, these values remained lower than those in the other two groups. On histological and immunohistochemical analysis, the chondrocytes were increased, and fewer matrix metalloproteinases-13 immunopositive cells were identified in the mono-iodoacetate + low-intensity pulsed ultrasound group than mono-iodoacetate group.
Low-intensity pulsed ultrasound for 2 weeks may have therapeutic potential for treating temporomandibular joint osteoarthritis lesions.
本研究评估低强度脉冲超声对成年大鼠颞下颌关节骨关节炎的作用。
通过小剂量单碘乙酸注射诱导 24 只成年大鼠颞下颌关节骨关节炎样病变。将大鼠分为 4 组：对照组和单碘乙酸组，分别于 12 周时注射对比剂和单碘乙酸，并观察至 20 周；低强度脉冲超声组和单碘乙酸+低强度脉冲超声组，分别于 12 周时注射对比剂和单碘乙酸，从 16 周开始至 20 周进行低强度脉冲超声治疗。每周用微计算机断层扫描评估髁状骨矿物质密度、骨矿物质含量和骨体积。20 周时行组织学和基质金属蛋白酶-13 免疫组织化学染色。
20 周时，单碘乙酸+低强度脉冲超声组的骨矿物质密度、骨矿物质含量和骨体积均明显高于单碘乙酸组，但仍低于其他两组。组织学和免疫组织化学分析显示，单碘乙酸+低强度脉冲超声组的软骨细胞增多，基质金属蛋白酶-13 免疫阳性细胞较单碘乙酸组减少。
2 周的低强度脉冲超声可能具有治疗颞下颌关节骨关节炎病变的潜力。
