He Zhi-Gang, Liu Bao-Wen, Li Zhi-Xiao, Tian Xue-Bi, Liu San-Guang, Manyande Anne, Zhang Ding-Yu, Xiang Hong-Bing
Department of Anesthesiology and Pain Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
Department of Hepatobiliary Surgery, The Second Hospital, Hebei Medical University, Shijiazhuang, People's Republic of China.
Oncotarget. 2017 May 18;8(42):71859-71866. doi: 10.18632/oncotarget.17983. eCollection 2017 Sep 22.
Understanding neuroanatomical sympathetic circuitry and neuronal connections from the caudal pedunculopontine tegmental nucleus to skeletal muscle is important to the study of possible mechanisms of pedunculopontine tegmental nucleus (PPTg) and cuneiform nucleus (CnF) that are involved in the regulation of skeletal muscle activity of the sympathetic pathway. The aim of this study was to use virus PRV-614 to trace the melanocortin-sympathetic neural pathways from PPTg and CnF to a hindlimb muscle (gastrocnemius) in spinally transected MC4R-GFP transgenic mice. PRV-614 was injected into the gastrocnemius muscle after receiving a complete spinal cord transection below the L2 level. PRV-614/MC4R-GFP and PRV-614/TPH dual-labeled neurons were found in the dissipated parts of PPTg (dpPPTg), but not between the compact parts of PPTg (cpPPTg) and CnF. It is proposed that a hierarchical pathway of neurons within the caudal pedunculopontine tegmental nucleus sends projections to the RVLM, which in turn projects onto the IML sympathetic preganglionic neurons that regulate muscle blood flow through melanocortin-sympathetic signals. Our results collectively indicate that MC4Rs expressed in caudal pedunculopontine tegmental nucleus may be involved in skeletal muscle activity of melanocortin-sympathetic pathways.
了解从尾侧脚桥被盖核到骨骼肌的神经解剖学交感神经回路和神经元连接,对于研究脚桥被盖核(PPTg)和楔形核(CnF)参与交感神经通路骨骼肌活动调节的可能机制具有重要意义。本研究的目的是利用病毒PRV - 614追踪脊髓横断的MC4R - GFP转基因小鼠中从PPTg和CnF到后肢肌肉(腓肠肌)的黑皮质素 - 交感神经通路。在L2水平以下接受完全脊髓横断后,将PRV - 614注入腓肠肌。在PPTg的分散部分(dpPPTg)发现了PRV - 614/MC4R - GFP和PRV - 614/TPH双标记神经元,但在PPTg的致密部分(cpPPTg)和CnF之间未发现。有人提出,尾侧脚桥被盖核内的神经元分级通路向延髓头端腹外侧网状核(RVLM)发出投射,RVLM进而投射到中间外侧柱交感神经节前神经元,这些神经元通过黑皮质素 - 交感神经信号调节肌肉血流。我们的结果共同表明,在尾侧脚桥被盖核中表达的MC4R可能参与黑皮质素 - 交感神经通路的骨骼肌活动。