在缅甸进行特征描述。
Characterizing in Myanmar.
机构信息
Department of Gastroenterology, Yangon General Hospital, University of Medicine (1), Yangon, Myanmar, Japan.
Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Japan.
出版信息
Gut Liver. 2018 Jan 15;12(1):51-57. doi: 10.5009/gnl17053.
BACKGROUND/AIMS: Differences in the infection rate are not sufficient to clarify the dissimilarity of gastric cancer incidence between Myanmar and its neighboring countries. To better understand this trend, the virulence gene was characterized in Myanmar.
METHODS
Glutamate-proline-isoleucine-tyrosine-alanine (EPIYA) patterns and CagA multimerization (CM) motifs of genotypes were examined by performing polymerase chain reactions and DNA sequencing.
RESULTS
Of 69 tested strains, -positive patients had significantly more severe histological scores in their antrum than -negative patients. Sequence analysis revealed that 94.1% of strains had Western-type containing an EPIYA motif (92.6%) or EPIYT motif (6.4%). The intestinal metaplasia scores in the antral of patients infected with the ABC and ABCC types of were significantly higher than those of patients with AB-type . Interestingly, in patients infected with , 46.3% of strains with three EPIYA motifs contained two identical Western-typical CM motifs, and these patients showed significantly higher antrum inflammation scores than patients infected with two identical nontypical-CM motif strains (p=0.02).
CONCLUSIONS
In Myanmarese strains, Western-type was predominant. The presence of CM motifs and the proportion of multiple EPIYA-C segments might partially explain the intermediate gastric cancer risk found in Myanmar.
背景/目的：感染率的差异不足以说明缅甸和其邻国之间胃癌发病率的差异。为了更好地了解这一趋势，我们对缅甸的毒力基因进行了特征分析。
方法
通过聚合酶链反应和 DNA 测序，检测基因型的谷氨酸-脯氨酸-异亮氨酸-酪氨酸-丙氨酸（EPIYA）模式和 CagA 多聚化（CM）基序。
结果
在 69 株检测的菌株中，阳性患者的胃窦组织学评分明显高于阴性患者。序列分析显示，94.1%的菌株含有 Western 型，包含 EPIYA 基序（92.6%）或 EPIYT 基序（6.4%）。感染 ABC 和 ABCC 型 的患者的胃窦肠化生评分明显高于 AB 型 感染患者。有趣的是，在感染 的患者中，46.3%的含有三个 EPIYA 基序的菌株含有两个相同的 Western 典型 CM 基序，这些患者的胃窦炎症评分明显高于感染两个相同非典型-CM 基序菌株的患者（p=0.02）。
结论
在缅甸菌株中，主要是 Western 型 。CM 基序的存在和多个 EPIYA-C 片段的比例可能部分解释了在缅甸发现的中等胃癌风险。
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