Zhong Zhijun, Dan Jiaming, Yan Guangwen, Tu Rui, Tian Yinan, Cao Suizhong, Shen Liuhong, Deng Junliang, Yu Shumin, Geng Yi, Gu Xiaobin, Wang Ya, Liu Haifeng, Peng Guangneng
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan 611130, PR China.
College of Animal Science, Xichang University, Xichang 615000, PR China.
Parasite. 2018;25:45. doi: 10.1051/parasite/2018046. Epub 2018 Sep 4.
Giardia duodenalis and Cryptosporidium spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for G. duodenalis and Cryptosporidium infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of G. duodenalis and Cryptosporidium spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for G. duodenalis and 40 (14.4%) were positive for Cryptosporidium spp. Cryptosporidium bovis (n = 28), Cryptosporidium ryanae (n = 5) and Cryptosporidium parvum (n = 7) were detected. All seven C. parvum isolates were successfully subtyped based on the gp60 gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of G. duodenalis based on beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize G. duodenalis in pre-weaned dairy calves in southwestern China.
十二指肠贾第虫和隐孢子虫属是常见的人和动物病原体。近年来，它们在犊牛中越来越多地被报道；然而，关于中国西南部感染未断奶犊牛的十二指肠贾第虫和隐孢子虫的多位点基因分型信息有限。在本研究中，测定了四川省中部未断奶犊牛中十二指肠贾第虫和隐孢子虫属的流行情况，并对病原体进行了分子分析。在278份未断奶犊牛的粪便样本中，26份（9.4%）十二指肠贾第虫检测呈阳性，40份（14.4%）隐孢子虫属检测呈阳性。检测到牛隐孢子虫（n = 28）、雷氏隐孢子虫（n = 5）和微小隐孢子虫（n = 7）。所有7株微小隐孢子虫分离株均基于gp60基因序列成功进行了亚型分型，仅检测到IIdA15G1。基于β-贾第虫（bg）、磷酸丙糖异构酶（tpi）和谷氨酸脱氢酶（gdh）基因的十二指肠贾第虫多位点序列分型揭示了19种不同的E群多位点基因型（2种已知基因型和17种未发表的基因型）。基于eBURST分析，在四川省未断奶犊牛中观察到E群内高度的遗传多样性。据我们所知，这是首次在中国西南部未断奶犊牛中使用多位点序列分型和eBURST分析来鉴定十二指肠贾第虫。
