Wu Zheng-Yu, Wang Ning-Ling, Liu Kang-Kang, Chu Jin-Hua, Wang Yan, Yang Lin-Hai, Xie Zhi-Wei
Department of Pediatrics, The Second Hospital of Anhui Medical University,Hefei 230061, Anhui Province,China.
Department of Pediatrics, The Second Hospital of Anhui Medical University,Hefei 230061, Anhui Province,China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Oct;25(5):1367-1372. doi: 10.7534/j.issn.1009-2137.2017.05.015.
Obejective: To investigate the expression level of suppressor of cytokine signaling SOCS3 mRNA in children's acute lymphoblastic leukemia(ALL); to analyze the relationship between the expresion level of SOCS3 mRNA and disease status and risks of ALL, and to explore the application of SOCS3 mRNA in evaluation of ALL disease status, risk and target therapy.
The expression levels of SOCS3 mRNA in bone marrow mononuclear cells from 45 cases of newly diagnosed ALL at initial diganosis and induction remission and 13 normal children as controls were detected by SYBR Green fluorescence quantitative PCR; the correlation of SOCS3 mRNA expression level with risk and clinical characteristics of ALL was analyzed by means of statistical method; the immunofluorescence histochemistry method and laser confocal microscopy were used to detect the sites and expression level of SOCS3 mRNA in bone marrow samples of ALL patients.
The SOCS3 mRNA expression level at initial diagnosis of 45 ALL patients was significantly lower than that of normal controls (P<0.05), and that in induction remission of 45 patients was not significant different from normal controls (P<0.05); the SOCS3 mRNA expression level at initial diagnosis of patients with standasd and high risk was higher than that of patients with low risk (P<0.05). The 45 patients were divided into high expression and low expression groups according to SOCS3 mRNA expression level at initial diagnosis by median method, comparison of clinical characteristics in 2 groups was found that the SOCS3 mRNA high expression group had even more higher leukocyte count in peripheral blood, even more higher LDH level and much more poor prognostic genes; in addition, the high expression group showed more higher risk in comparison between 2 group.
The SOCS3 mRNA expression is down-regulated at initial diagnosis, while recovered to normal level after induction remission of disease, thus the SOCS3 can be used as an indicator for evaluation of disease status. The high expression of SOCS3 mRNA up-regulates the disease risk, therefore the SOCS3 mRNA can be used as a factor for evaluation of ALL risk. The treatment of ALL via regulation of SOCS3 mRNA expression level maybe can become a new way. The regulation of SOCS3 mRNA expression level maybe can become a new way for treatment of ALL.
目的：研究细胞因子信号抑制因子SOCS3 mRNA在儿童急性淋巴细胞白血病（ALL）中的表达水平；分析SOCS3 mRNA表达水平与ALL疾病状态及风险的关系，探讨SOCS3 mRNA在评估ALL疾病状态、风险及靶向治疗中的应用。
采用SYBR Green荧光定量PCR检测45例初诊及诱导缓解期新诊断ALL患儿骨髓单个核细胞中SOCS3 mRNA的表达水平，并以13例正常儿童作为对照；采用统计学方法分析SOCS3 mRNA表达水平与ALL风险及临床特征的相关性；采用免疫荧光组织化学法及激光共聚焦显微镜检测ALL患者骨髓样本中SOCS3 mRNA的表达部位及表达水平。
45例ALL患者初诊时SOCS3 mRNA表达水平显著低于正常对照组（P<0.05），45例患者诱导缓解期与正常对照组比较差异无统计学意义（P<0.05）；标准及高危患者初诊时SOCS3 mRNA表达水平高于低危患者（P<0.05）。将45例患者按初诊时SOCS3 mRNA表达水平采用中位数法分为高表达组和低表达组，比较两组临床特征发现，SOCS3 mRNA高表达组外周血白细胞计数更高、乳酸脱氢酶水平更高且预后不良基因更多；此外，两组比较高表达组风险更高。
SOCS3 mRNA在初诊时表达下调，疾病诱导缓解后恢复至正常水平，因此SOCS3可作为评估疾病状态的指标。SOCS3 mRNA高表达上调疾病风险，因此SOCS3 mRNA可作为评估ALL风险的因素。通过调节SOCS3 mRNA表达水平治疗ALL可能成为一种新途径。调节SOCS3 mRNA表达水平可能成为治疗ALL的新途径。
