Xu He-Ming, Xie Ze-Xiong, Liu Duo, Wu Yi, Li Bing-Zhi, Yuan Ying-Jin
1. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072,China; 2. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.
Yi Chuan. 2017 Oct 20;39(10):865-876. doi: 10.16288/j.yczz.17-199.
With the rapid growth and development of synthetic biology, research in the genomics is advancing from genome sequencing to genome synthesis. In 2009, Professor Jef D. Boeke proposed the Synthetic Yeast Genome Project (Sc2.0), which aims to synthesize the world's first eukaryotic genome. With the efforts of scientists from the United States, China, Britain, France, Australia, Singapore and other countries, a third of the Saccharomyces cerevisiae chromosomes has now been synthesized. In the perspectives of synthetic genomics, we here review the recent progress in the Sc2.0 project, including discussion on the right arm of chromosome 9, and chromosomes 2, 5, 6, 10, 12, in terms of their designs and synthetic strategy as well as the biological significance, thereby providing a reference for further research in synthetic genomics.
随着合成生物学的快速发展，基因组学研究正从基因组测序迈向基因组合成。2009年，杰夫·D·伯克教授提出了合成酵母基因组计划（Sc2.0），旨在合成世界上首个真核生物基因组。在美国、中国、英国、法国、澳大利亚、新加坡等国科学家的努力下，目前已合成了酿酒酵母三分之一的染色体。从合成基因组学的角度，我们在此综述Sc2.0计划的最新进展，包括对9号染色体右臂以及2号、5号、6号、10号、12号染色体在设计、合成策略及生物学意义方面的讨论，从而为合成基因组学的进一步研究提供参考。
