人工合成酵母基因组的设计。

Design of a synthetic yeast genome.

机构信息

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.

High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

出版信息

Science. 2017 Mar 10;355(6329):1040-1044. doi: 10.1126/science.aaf4557.

DOI:10.1126/science.aaf4557

PMID:28280199

Abstract

We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.

摘要

我们描述了一个合成真核基因组 Sc2.0 的完整设计，这是一个经过高度修饰的基因组，大小减少了近 8%，其中有 110 万个碱基对被删除、插入或改变。Sc2.0 染色体的设计是使用 BioStudio 实现的，这是一个为真核基因组设计开发的开源框架，它协调从核苷酸到基因组尺度的设计修改，并实施版本控制，以系统地跟踪编辑。为了实现完整的 Sc2.0 基因组合成，世界各地的 Sc2.0 联盟团队构建的单个合成染色体将通过“内复制杂交”整合到一个单一的菌株中。像 Sc2.0 这样的化学合成基因组是完全可定制的，允许实验人员采用自下而上的设计策略，提出关于染色体结构、功能和进化的其他难以解决的问题。