Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan.
Department of Urology, St. Marianna University School of Medicine, 2-16-1, Sugao, Miyamae-ku, Kawasaki, Kanagawa, 216-8511, Japan.
Sci Rep. 2017 Oct 25;7(1):14036. doi: 10.1038/s41598-017-14492-9.
Previous studies have investigated the use of mesenchymal stem cells (MSCs) to treat damaged kidneys. However, the effect of adipose-derived MSCs (ASCs) on vascular calcification in chronic kidney disease (CKD) is still poorly understood. In the present study, we explored the potential of ASCs for the treatment of CKD and vascular calcification. CKD was induced in male Sprague-Dawley rats by feeding them a diet containing 0.75% adenine for 4 weeks. ASCs transplantation significantly reduced serum inorganic phosphorus (Pi) as compared to that in the control. The histopathology of the kidneys showed a greater dilation of tubular lumens and interstitial fibrosis in the control group. Calcium and Pi contents of the aorta in the ASCs transplantation group were lower than those in the control group. Von Kossa staining of the thoracic aorta media revealed that ASCs transplantation suppressed vascular calcification. Thus, this study revealed that autogenic ASCs transplantation inhibits kidney damage and suppresses the progression of vascular calcification in the CKD rat model, suggesting that autogenic ASCs transplantation is a novel approach for preventing the progression of CKD and vascular calcification.
先前的研究已经探讨了间充质干细胞（MSCs）在治疗受损肾脏方面的应用。然而，脂肪来源的间充质干细胞（ASCs）对慢性肾脏病（CKD）中血管钙化的影响仍知之甚少。在本研究中，我们探索了 ASCs 治疗 CKD 和血管钙化的潜力。通过给予雄性 Sprague-Dawley 大鼠含有 0.75%腺嘌呤的饮食 4 周，诱导 CKD。与对照组相比，ASCs 移植显著降低了血清无机磷（Pi）。肾脏的组织病理学显示对照组肾小管腔和间质纤维化明显扩张。ASCs 移植组的主动脉钙和 Pi 含量低于对照组。胸主动脉中膜的 von Kossa 染色显示，ASCs 移植抑制了血管钙化。因此，这项研究表明，自体 ASCs 移植抑制了 CKD 大鼠模型中的肾脏损伤，并抑制了血管钙化的进展，表明自体 ASCs 移植是预防 CKD 和血管钙化进展的一种新方法。
