Chen Chun-Nan, Chen You-Tzung, Yang Tsung-Lin
Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Data Brief. 2017 Sep 28;15:545-561. doi: 10.1016/j.dib.2017.09.053. eCollection 2017 Dec.
Tumor satellite formation is an indicator of cancer invasiveness and correlates with recurrence, metastasis, and poorer prognosis. By analyzing pathological specimens, tumor satellites formed at the tumor-host interface reflect the phenomena of epithelial-mesenchymal transition. It is impossible to reveal the dynamic processes and the decisive factors of tumor satellite formation using clinicopathological approaches alone. Therefore, establishment of an system to monitor the phenomena is important to explicitly elucidate underlying mechanisms. In this study, we explored the feasibility of creating an three-dimensional collagen culture system to recapitulate the process of tumor satellite formation. This data presented here are referred to the research article (Chen et al., 2017) [1]. Using this model, the dynamic process of tumor satellite formation could be recapitulated in different types of human cancer cells. Induced by calcium deprivation, the treated cells increased the incidence and migratory distance of tumor satellites. E-cadherin internalization and invadopodia formation were enhanced by calcium deprivation and were associated with cellular dynamic change during tumor satellite formation. The data confirmed the utility of this culture system to recapitulate dynamic cellular alteration and to explore the potential mechanisms of tumor satellite formation.
肿瘤卫星灶形成是癌症侵袭性的一个指标，与复发、转移及较差的预后相关。通过分析病理标本，在肿瘤-宿主界面形成的肿瘤卫星灶反映了上皮-间质转化现象。仅使用临床病理方法无法揭示肿瘤卫星灶形成的动态过程及决定性因素。因此，建立一个监测该现象的系统对于明确阐明潜在机制很重要。在本研究中，我们探讨了创建一个三维胶原培养系统以重现肿瘤卫星灶形成过程的可行性。此处呈现的数据参考了研究文章（Chen等人，2017年）[1]。使用该模型，不同类型的人类癌细胞中均可重现肿瘤卫星灶形成的动态过程。钙剥夺诱导后，处理后的细胞增加了肿瘤卫星灶的发生率和迁移距离。钙剥夺增强了E-钙黏蛋白内化和侵袭性伪足形成，并与肿瘤卫星灶形成过程中的细胞动态变化相关。这些数据证实了该培养系统在重现动态细胞改变及探索肿瘤卫星灶形成潜在机制方面的实用性。
