Hernández-Fernández Javier
Genetics, Molecular Biology and Bioinformatics Lab, Department of Natural and Environmental Sciences, Universidad Jorge Tadeo Lozano, Cra. 4 N° 22-61 modulo 7 Piso 6, Bogotá D.C., Colombia.
Data Brief. 2017 Oct 11;15:573-576. doi: 10.1016/j.dib.2017.10.015. eCollection 2017 Dec.
The hawksbill sea turtle, is an endangered species of the Caribbean Colombian coast due to anthropic and natural factors that have decreased their population levels. Little is known about the genes that are involved in their immune system, sex determination, aging and others important functions. The data generated represents RNA sequencing and the first de-novo assembly of transcripts expressed in the blood of the hawksbill sea turtle. The raw FASTQ files were deposited in the NCBI SRA database with accession number SRX2653641. A total of 5.7 Gb raw sequence data were obtained, corresponding to 47,555,108 raw reads. Trinity was used to perform a first de-novo assembly, and we were able to identify 47,586 transcripts of the female hawksbill turtle transcriptome with an N50 of 1100 bp. The obtained transcriptome data will be useful for further studies of the physiology, biochemistry and evolution in this species.
玳瑁海龟是加勒比哥伦比亚海岸的濒危物种，由于人为和自然因素，其种群数量有所减少。人们对其免疫系统、性别决定、衰老及其他重要功能所涉及的基因知之甚少。所生成的数据代表RNA测序以及玳瑁海龟血液中表达的转录本的首次从头组装。原始FASTQ文件已存入NCBI SRA数据库，登录号为SRX2653641。共获得5.7Gb原始序列数据，对应47,555,108条原始读数。使用Trinity进行首次从头组装，我们能够鉴定出雌性玳瑁海龟转录组的47,586个转录本，N50为1100bp。所获得的转录组数据将有助于对该物种的生理学、生物化学和进化进行进一步研究。
