Department of Clinical Laboratory, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Clinical Laboratory, Beijing Jishuitan Hospital, Peking University, Beijing, China.
Probiotics Antimicrob Proteins. 2018 Jun;10(2):236-242. doi: 10.1007/s12602-017-9339-x.
Lactobacilli have been shown to inhibit the proliferation of several types of cancer cells, but the effects of vaginal Lactobacilli on cervical cancer cells have seldom been reported. We incubated Caski cells with supernatants of predominant strains in the vagina and investigated their effects on cell growth and the possible mechanisms. Cell-free supernatants of Lactobacillus crispatus, L. jensenii, and L. gasseri were prepared and purified. Caski cells were treated with various concentrations of Lactobacillus supernatants (LS). The effect of LS on cell growth was investigated using MTT assays. The influence of LS on the cell cycle and expression of human papillomavirus (HPV) E6 and E7 oncogenes was determined by flow cytometry and RT-PCR, respectively. LS-inhibited Caski cell proliferation caused morphological changes in a pH-independent manner. Flow cytometric analysis revealed that cells exposed to LS exhibited a significant increase of cell number in S phase and a strong decrease of cell number in G2/M phase. Expression of HPV E6 and E7 oncogenes, as well as CDK2 and cyclin A was decreased after treatment with LS, while expression of p21 was increased. Supernatants of L. crispatus, L. jensenii, and L. gasseri have inhibitory effects on the viability of cervical cancer cells via regulation of HPV oncogenes and cell cycle-related genes. Lactobacillus, as a promising treatment for cancer, is being assessed for its effect, and these results provide further evidence in this respect.
乳酸杆菌已被证明能抑制多种类型的癌细胞增殖，但阴道乳酸杆菌对宫颈癌的作用鲜有报道。我们将宫颈癌细胞与阴道中优势菌的上清液共孵育，研究其对细胞生长的影响及其可能的机制。制备并纯化了詹氏乳杆菌、约氏乳杆菌和加氏乳杆菌的无细胞上清液。用不同浓度的乳酸杆菌上清液（LS）处理 Caski 细胞。用 MTT 法检测 LS 对细胞生长的影响。用流式细胞术和 RT-PCR 分别测定 LS 对细胞周期和人乳头瘤病毒（HPV）E6 和 E7 癌基因表达的影响。LS 抑制 Caski 细胞增殖导致细胞形态发生 pH 独立的变化。流式细胞术分析显示，LS 作用的细胞 S 期细胞数明显增加，G2/M 期细胞数明显减少。用 LS 处理后，HPV E6 和 E7 癌基因以及 CDK2 和细胞周期蛋白 A 的表达降低，而 p21 的表达增加。詹氏乳杆菌、约氏乳杆菌和加氏乳杆菌的上清液通过调节 HPV 癌基因和细胞周期相关基因对宫颈癌细胞的活力有抑制作用。乳酸杆菌作为一种有前途的癌症治疗方法，其效果正在评估中，这些结果为此提供了进一步的证据。
