Bruce Bagwell C
Verity Software House, P.O. Box 247, 45A Augusta Road, Topshsam, ME, 04086, USA.
Methods Mol Biol. 2018;1678:11-36. doi: 10.1007/978-1-4939-7346-0_2.
This chapter outlines how to approach the complex tasks associated with designing models for high-dimensional cytometry data. Unlike gating approaches, modeling lends itself to automation and accounts for measurement overlap among cellular populations. Designing these models is now easier because of a new technique called high-definition t-SNE mapping. Nontrivial examples are provided that serve as a guide to create models that are consistent with data.
本章概述了如何处理与高维细胞计数数据模型设计相关的复杂任务。与门控方法不同，建模便于自动化操作，并能处理细胞群体间的测量重叠问题。由于一种名为高清t-SNE映射的新技术，现在设计这些模型变得更加容易。文中提供了一些重要示例，可作为创建与数据相符模型的指南。
