多参数传统流式细胞术
Multiparameter Conventional Flow Cytometry.
作者信息
McKinnon Katherine M
机构信息
Vaccine Branch Flow Cytometry Facility, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 41, Room B715, 9000 Rockville Pike, Bethesda, MD, 20892, USA.
出版信息
Methods Mol Biol. 2018;1678:139-150. doi: 10.1007/978-1-4939-7346-0_8.
Multicolor flow cytometry is a useful technique when examining mixed populations of cells, such as blood and tissue cells in human and animal samples. The ability to use multiple fluorescent markers simultaneously allows for the identification of multiple cell types, as well as functional markers that further characterize each sample. The introduction of instruments capable of measuring 12-plus colors and new reagents has made this type of flow cytometry both popular and problematic. Adapting a typical staining panel from 4 to 6 color tubes to more than 12 colors is not simply a matter of "plug and play", but must be approached in a systematic manner to achieve a successful multi-parameter staining panel. This chapter will examine the considerations and methods needed to successfully perform multicolor flow cytometry.
多色流式细胞术在检测混合细胞群体时是一项有用的技术，比如人和动物样本中的血液和组织细胞。同时使用多种荧光标记物的能力使得能够识别多种细胞类型以及进一步表征每个样本的功能标记物。能够测量12种以上颜色的仪器和新试剂的引入，使得这种类型的流式细胞术既流行又存在问题。将一个典型的4至6色管染色方案调整为12种以上颜色，并非简单的“即插即用”，而必须以系统的方式进行，以实现成功的多参数染色方案。本章将探讨成功进行多色流式细胞术所需考虑的因素和方法。
Zotero 插件