[电针“足三里”（ST 36）对脾虚证大鼠空肠中胃饥饿素/cAMP/PKA表达的影响]

[Effect of Electroacupuncture at "Zusanli"(ST 36) on the Expression of Ghrelin/cAMP/PKA in the Jejunum in Rats with Spleen Deficiency Syndrome].

作者信息

Yong Ru-Lin, Qu Yi, Li Xin-Xin, Wang Jian-Bo, Xue Ya-Nan, Zhang Li-de

机构信息

Key Laboratory of Chinese Mi-nistry of Education for Chinese Medical Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.

出版信息

Zhen Ci Yan Jiu. 2016 Dec 25;41(6):497-501.

PMID:29071891

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) at "Zusanli"(ST 36) on Ghrelin/cAMP/PKA expression in the jejunum in rats with spleen deficiency syndrome, so as to reveal its underlying mechanism in improving energy metabolism.

METHODS

Forty male SD rats were randomly divided into 4 groups:normal group, spleen deficiency syndrome (model) group, EA group and non-acupoint group (=10 in each group).The model of spleen deficiency syndrome was established by improper diet and overstrain. EA (2 Hz/15 Hz, 0.5 mA) was applied to bilateral "Zusanli" (ST 36) in the EA group and non-acupoint in non-acupoint group for 20 min, once a day for 6 days. The pathologic changes of the jejunum tissue were detected by H&E staining. Ghrelin, ATP and cAMP levels in jejunum tissue were determined by ELISA. The expression levels of PKA protein in jejunum tissue were determined by Western blot.

RESULTS

H&E staining showed that the intestinal villi of the model group were swelling, shortening and thickening, with a damaged or broken top-part in the model group, and basically restored to normal after EA treatment. ELISA results showed that the contents of Ghrelin, ATP and cAMP in the jejunum tissue were significantly lower in the model group than in the normal group (<0.05), while significantly higher in the EA group than in the model group (<0.05). Western blot results showed that the expression of PKA protein in the jejunum tissue was significantly lower in the model group than in the normal group (<0.05), and significantly higher in the EA group than in the model group and non-acupoint group (<0.05).

CONCLUSIONS

EA at ST 36 can improve the morphological changes in the jejunum of spleen deficiency rats, which may be associated with its effects in increasing Ghrelin, ATP and cAMP contents, and up-regulating PKA expression, leading to an increase of energy metabolism and spleen at last.

摘要

目的

观察电针“足三里”（ST 36）对脾虚证大鼠空肠中胃饥饿素/环磷酸腺苷/蛋白激酶A（Ghrelin/cAMP/PKA）表达的影响，以揭示其改善能量代谢的潜在机制。

方法

将40只雄性SD大鼠随机分为4组：正常组、脾虚证（模型）组、电针组和非穴位组（每组10只）。通过饮食不当和过度劳累建立脾虚证模型。电针组将电针（2 Hz/15 Hz，0.5 mA）施加于双侧“足三里”（ST 36），非穴位组将电针施加于非穴位，持续20分钟，每天1次，共6天。采用苏木精-伊红（H&E）染色检测空肠组织的病理变化。采用酶联免疫吸附测定（ELISA）法测定空肠组织中胃饥饿素、三磷酸腺苷（ATP）和环磷酸腺苷（cAMP）水平。采用蛋白质免疫印迹法（Western blot）测定空肠组织中蛋白激酶A（PKA）蛋白的表达水平。

结果

H&E染色显示，模型组肠绒毛肿胀、缩短、增粗，顶端部分受损或断裂，电针治疗后基本恢复正常。ELISA结果显示，模型组空肠组织中胃饥饿素、ATP和cAMP含量显著低于正常组（P<0.05），而电针组显著高于模型组（P<0.05）。Western blot结果显示，模型组空肠组织中PKA蛋白表达显著低于正常组（P<0.05），电针组显著高于模型组和非穴位组（P<0.05）。