Chen Yan-Ping
Department of Rehabilitation, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471000, China.
Zhen Ci Yan Jiu. 2016 Jun 25;41(3):210-4.
To observe the effect of moxibustion and electroacupuncture (EA) stimulation of "Tianshu" (ST 25) and "Qihai" (CV 6) on Th 1/Th 2 immune balance in rats with experimental ulcerative colitis (UC).
The UC rats were randomly allocated to model group, moxibustion group and EA group (=10 in each group). Another 10 rats were used as the control group. The UC model was established by injection of fresh colonic mucosal tissue-made antigen emulsifier (purified mucosal supernate plus complete Freund's adjuvant, 3.5 mg on day one, and 7 mg thereafter) into the bilateral toes, inguinal grooves, back, and the abdominal cavity on day 1, 10, 17 and 24, respectively. Medicinal paste (containing etc.)-separated moxibustion or EA (200 Hz, 2 mA) was applied to ST 25 and CV 6 for 20 min, once daily for 14 days. The mRNA expression levels of interferon-γ (INF-γ), IL-12, IL-4 and IL-10 in colon tissues were detected using quantitative RT-PCR and their content determined using enzyme linked immunosorbent assay (ELISA). The colon tissue cell ratios of CD 4 INF-γ and CD 4 IL-4 were analyzed by flow cytometry.
Compared with the control group, the mRNA and contents of colonic INF-γ and IL-12 and the ratio of CD 4 INF-γ and CD 4 IL-4 were significantly higher in the model group (<0.05), and the IL-4 and IL-10 mRNA expression and contents considerably lower after UC modeling (<0.05). Following medicinal paste-separated moxibustion and EA interventions, the increased INF-γ and IL-12 mRNA and contents and the increased ratio of CD 4 INF-γ and CD 4 IL-4, and the decreased IL-4 and IL-10 mRNA and contents were reversed (<0.05). No significant differences were found between the moxibustion and EA groups in the levels of all the indexes (>0.05).
Both medicinal paste-separated moxibustion and EA interventions may regulate the balance between Th 1 and Th 2 cells in the colonic tissues of UC rats, suggesting an improvement of the immune function by down-regulating INF-γ and IL-12 levels and up-regulating IL-4 and IL-10 levels.
观察艾灸及电针刺激“天枢”（ST 25）和“气海”（CV 6）对实验性溃疡性结肠炎（UC）大鼠Th1/Th2免疫平衡的影响。
将UC大鼠随机分为模型组、艾灸组和电针组（每组各10只）。另取10只大鼠作为对照组。于第1、10、17和24天分别将新鲜结肠黏膜组织制成的抗原乳化剂（纯化的黏膜上清液加完全弗氏佐剂，第1天3.5 mg，此后7 mg）注射到双侧足趾、腹股沟、背部及腹腔内，建立UC模型。将药饼（含……等）隔物艾灸或电针（200 Hz，2 mA）施于ST 25和CV 6，每次20分钟，每日1次，共14天。采用定量RT-PCR检测结肠组织中干扰素-γ（INF-γ）、IL-12、IL-4和IL-10的mRNA表达水平，并用酶联免疫吸附测定（ELISA）法测定其含量。通过流式细胞术分析结肠组织中CD4 INF-γ和CD4 IL-4的细胞比例。
与对照组相比，模型组结肠INF-γ和IL-12的mRNA及含量、CD4 INF-γ和CD4 IL-4的比例显著升高（<0.05），UC建模后IL-4和IL-10的mRNA表达及含量显著降低（<0.05）。经过药饼隔物艾灸和电针干预后，升高的INF-γ和IL-12的mRNA及含量、升高的CD4 INF-γ和CD4 IL-4的比例以及降低的IL-4和IL-10的mRNA及含量均得到逆转（<0.05）。艾灸组和电针组各指标水平比较差异无统计学意义（>0.05）。
药饼隔物艾灸和电针干预均可能调节UC大鼠结肠组织中Th1和Th2细胞之间的平衡，提示通过下调INF-γ和IL-12水平、上调IL-4和IL-10水平改善免疫功能。
