Yang Meng, Li Li-Hong, Wang Di, Hou Xun-Rui, Zhang Yuan
Clinical Medical College of Guizhou Medical University, Guiyang 550004, China.
The Affliated Hospital of Guizhou Medical University, Guiyang 550004.
Zhen Ci Yan Jiu. 2016 Jun 25;41(3):220-4.
To observe the therapeutic effect of acupoint injection of mixture solution of lidocaine, dexa-methasone (DX) and transfer factor on symptoms and serum histamine contents in allergic rhinitis (AR) rats, so as to differentiate the role of acupoint stimulation or drug factor influencing the therapeutic effect.
SD rats were randomized into normal (=8), model, non-acupoint-drug injection, acupoint-drug injection and acupoint-saline injection groups (=11 in each group). The AR model was established by intraperitoneal injection of suspension of ovalbumin (0.3 mg) and aluminum hydroxide (30 mg), 1 mL, once every other day for 7 days, followed by nose-dripping of 5% ovalbumin (50 μL) for other 7 days. The AR scores for rhinocnesmus severity (1-2 points), sneezing times (1-3 points) and nasal discharge quantity (1-3 points) were assessed by cumulative quantification scoring method. "Yintang" (EX-HN 3) and bilateral "Yingxiang" (LI 20) were used for acupoint injection of mixture solution of 1% Lidocaine, DX and transfer factor (1:1:1, 0.1 mL/acupoint), once every other day for 7 days. The non-acupoints were located at the bilateral sub-costal regions and the mid-point between the left "Houhai" (GV 1) and "Huantiao" (GB 30).The same dose of saline was injected into the aforementioned acupoints for control. Serum histamine content was detected by using ELISA, and the nasal mucosal pathological changes were also observed after H.E. stain.
After the treatment, AR modeling induced increase of symptom scores and serum histamine contents were significantly decreased in both acupoint-saline injection and acupoint-drug injection groups (<0.01) but not in the non-acupoint-drug injection group (>0.05), and the effects of acupoint-drug injection were markedly superior to those of non-acupoint-drug injection group (<0.01).
Acupoint injection of mixture solution of Lidocaine, DX and transfer factor is effective in relieving the allergic symptoms and reducing the nasal mucosa inflammation in AR rats, which may be related to its action in suppressing histamine releasing, mainly by acupoint stimulation. It suggests that the therapeutic effect mainly derives from acupoint-injection stimulation, then the injected drugs.
观察利多卡因、地塞米松（DX）和转移因子混合液穴位注射对变应性鼻炎（AR）大鼠症状及血清组胺含量的影响，以区分穴位刺激或药物因素对治疗效果的作用。
将SD大鼠随机分为正常组（n = 8）、模型组、非穴位药物注射组、穴位药物注射组和穴位生理盐水注射组（每组n = 11）。通过腹腔注射卵清蛋白（0.3 mg）和氢氧化铝（30 mg）混悬液1 mL，隔日1次，共7天，随后滴鼻5%卵清蛋白（50 μL），共7天，建立AR模型。采用累积定量评分法评估鼻痒严重程度（1 - 2分）、喷嚏次数（1 - 3分）和鼻分泌物量（1 - 3分）的AR评分。取“印堂”（EX-HN 3）及双侧“迎香”（LI 20）进行穴位注射1%利多卡因、DX和转移因子混合液（1:1:1，0.1 mL/穴），隔日1次，共7天。非穴位位于双侧肋下区及左侧“后海”（GV 1）与“环跳”（GB 30）连线中点。上述穴位注射相同剂量生理盐水作为对照。采用ELISA法检测血清组胺含量，HE染色后观察鼻黏膜病理变化。
治疗后，穴位生理盐水注射组和穴位药物注射组AR造模诱导的症状评分增加及血清组胺含量均显著降低（P < 0.01），而非穴位药物注射组无明显变化（P > 0.05），且穴位药物注射组的效果明显优于非穴位药物注射组（P < 0.01）。
利多卡因、DX和转移因子混合液穴位注射可有效缓解AR大鼠的变应性症状，减轻鼻黏膜炎症，这可能与其抑制组胺释放的作用有关，主要是通过穴位刺激实现。提示治疗效果主要源于穴位注射刺激，其次是注入的药物。
