Liang Fei-Hong, Hou Xun-Rui, Li Li-Hong, Liang Xin, Lu Yu-Wei, Yang Hong, Zhang Yuan
Clinical Medical College, Guizhou Medical University, Guiyang 550004, China.
the Affliated Hospital, Guizhou Medical University, Guiyang 550004, China.
Zhen Ci Yan Jiu. 2018 Apr 25;43(4):231-5. doi: 10.13702/j.1000-0607.170791.
To observe the effect of acupoint injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) on nasal allergic reactions and the expression of histamine receptor H 1 and H 4 in nasal mucosa of allergic rhinitis (AR) rats, so as to reveal its mechanism underlying improvement of AR.
SD rats were randomized into normal, model, non-acupoint injection and acupoint injection groups (=8 in each). The AR model was established by intraperitoneal (i.p.) injection of mixture of ovalbumin, aluminium hydroxide gel and normal saline (once every other day, for 7 times), and nasal drip of ovalbumin (on the following day of i.p.for 7 days). The mixture solution of lidocaine, dexamethasone (DXM) and transfer factor (0.1 mL/acupoint or non-acupoint) was injected into bilateral LI 20 and GV 29 in the acupoint injection group, or into the non-acupoints (about 5 cm below the armpit on both sides, and the middle point between the left "Houhai"[GV 1] and "Huantiao"[GB 30]), on the 1 , 5, 9, and 13 day after modeling. Symptoms of sneezing, nasal discharge, nose-rubbing, etc. were scored after the treatment. The expression levels of H1 R and H4 R proteins and genes in the nasal mucosa tissue were determined using immunohistochemistry and quantitative real-time PCR, respectively.
Compared with the normal group, the symptom score and the expression levels of H R, H R proteins and genes in nasal mucosa were significantly increased in the model group (<0.05). After acupoint injection for 4 times, the symptom score and the expression levels of H R, H R proteins and mRNAs were significantly down-regulated in the acupoint injection group relevant to the model group (<0.05). There were no significant differences between the non-acupoint and the model groups in the abovementioned indexes (>0.05), suggesting a specificity of the effect of acupoint injection.
Acupoint injection can relieve the allergic symptoms of AR rats, which may be related to its effects in down-regulating the over expression of H R and H R proteins and genes in the nasal mucosa.
观察针刺“迎香”（LI 20）、“印堂”（GV 29）穴对变应性鼻炎（AR）大鼠鼻黏膜过敏反应及组胺H1、H4受体表达的影响，以揭示其改善AR的作用机制。
将SD大鼠随机分为正常组、模型组、非穴位注射组和穴位注射组（每组8只）。采用腹腔注射卵白蛋白、氢氧化铝凝胶和生理盐水的混合液（隔日1次，共7次），并滴鼻给予卵白蛋白（腹腔注射次日开始，共7天）建立AR模型。穴位注射组于造模后第1、5、9、13天，将利多卡因、地塞米松（DXM）和转移因子的混合液（0.1 mL/穴或非穴位）注入双侧LI 20、GV 29穴，非穴位注射组则注入非穴位（双侧腋窝下约5 cm处及左侧“后海”[GV 1]与“环跳”[GB 30]连线中点）。治疗后对喷嚏、流涕、蹭鼻等症状进行评分。分别采用免疫组化和实时荧光定量PCR法检测鼻黏膜组织中H1R和H4R蛋白及基因的表达水平。
与正常组比较，模型组症状评分及鼻黏膜H1R、H4R蛋白及基因表达水平均显著升高（P<0.05）。穴位注射4次后，穴位注射组症状评分及H1R、H4R蛋白及mRNA表达水平与模型组比较均显著下调（P<0.05）。非穴位注射组与模型组上述指标比较差异无统计学意义（P>0.05），提示穴位注射效应具有特异性。
穴位注射可缓解AR大鼠的过敏症状，其机制可能与下调鼻黏膜H1R、H4R蛋白及基因的过表达有关。
