[中药膏剂穴位敷贴通过下调变应性鼻炎大鼠血清转化生长因子-β1缓解症状及鼻黏膜炎症反应]

[Acupoint application of herbal paste relieves symptoms and naso-mucosal inflammatory response by down-regulating serum TGF-β1 in allergic rhinitis rats].

作者信息

Chen Shan, Jin Yu-Tong, Zhu Zheng-Yang, Wu Ling-Tao, Xuan Li-Hua

机构信息

Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Zhejiang University of Chinese Medicine, Hangzhou 310006, China; The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053.

The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053.

出版信息

Zhen Ci Yan Jiu. 2019 Jun 25;44(6):430-3. doi: 10.13702/j.1000-0607.180369.

DOI:10.13702/j.1000-0607.180369

PMID:31368266

Abstract

OBJECTIVE

To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis (AR), serum immunoglobulin E (IgE) and transforming growth factor beta 1 (TGF-β1) level, and number of nasal eosinophils (EOS) in rats with AR, so as to explore its underlying mechanisms.

METHODS

Forty male Wistar rats were randomly divided into normal control, model, medication and acupoint application groups (＝10 rats per group). The AR model was established by intraperitoneal (i.p.) injection of mixture solution of ovalbumin, aluminum hydroxide and normal saline (once every other day, for 7 times), and nasal drip plus spray inhalation of ovalbumin (on the following day of i．p., once daily for 9 days). For acupoint application, the prepared herbal paste (containing , unprocessed and ginger juice) was applied to bilateral "Feishu" (BL13), "Pishu" (BL20) and "Shenshu" (BL23) for 2 h, once every other day for 7 times. The rats in the medication group were given Fluticasone Propionate nasal spray daily for 14 days. Scores of nasal itching, sneezing and nasal discharge on the day after modeling and the ending of the intervention were used to evaluate behavioral changes. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of serum IgE and TGF-β1, and the infiltration state of EOS in the nasal mucosa tissue was observed under light microscope after HE staining.

RESULTS

After modeling and compared with the normal control group, the behavioral scores and the levels of serum IgE and TGF-β1 were significantly higher (<0.05), and the infiltration state of EOS got worse. Compared with the model group, the increased behavioral score and serum IgE and TGF-β1 levels were evidently suppressed (<0.05) and EOS infiltration severity in the nasal mucosa was obviously milder in both medication and acupoint application groups. No significant differences were found between the medication and acupoint application groups in behavioral score and serum IgE and TGF-β1 levels (>0.05).

CONCLUSION

Acupoint application can improve the symptoms of AR rats, which may be associated with its effect in down-regulating the levels of serum IgE and TGF-β1.

摘要

目的

观察中药膏剂穴位贴敷对变应性鼻炎（AR）大鼠症状、血清免疫球蛋白E（IgE）及转化生长因子β1（TGF-β1）水平和鼻黏膜嗜酸性粒细胞（EOS）数量的影响，以探讨其作用机制。

方法

将40只雄性Wistar大鼠随机分为正常对照组、模型组、药物治疗组和穴位贴敷组（每组10只）。采用腹腔注射卵白蛋白、氢氧化铝和生理盐水混合液（隔日1次，共7次），并滴鼻加喷雾吸入卵白蛋白（腹腔注射次日开始，每日1次，共9天）的方法建立AR模型。穴位贴敷时，将制备好的中药膏剂（含、生、姜汁）贴敷于双侧“肺俞”（BL13）、“脾俞”（BL20）和“肾俞”（BL23），每次2小时，隔日1次，共7次。药物治疗组大鼠每日给予丙酸氟替卡松鼻喷雾剂，共14天。分别于造模后及干预结束后次日观察大鼠鼻痒、喷嚏及流涕症状评分，以评估行为学变化。采用酶联免疫吸附测定（ELISA）法检测血清IgE和TGF-β1水平，HE染色后光镜下观察鼻黏膜组织EOS浸润情况。

结果

造模后，与正常对照组比较，模型组大鼠行为学评分及血清IgE、TGF-β1水平显著升高（<0.05），EOS浸润加重。与模型组比较，药物治疗组和穴位贴敷组大鼠行为学评分及血清IgE、TGF-β1水平升高均明显受到抑制（<0.05），鼻黏膜EOS浸润程度明显减轻。药物治疗组和穴位贴敷组在行为学评分及血清IgE、TGF-β1水平方面比较，差异无统计学意义（>0.05）。