Xu Lei, Yan Xing-Zhou, Li Zhen-Yu, Cao Xiao-Fang, Wang Min
The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui Province, China.
Zhen Ci Yan Jiu. 2017 Jun 25;42(3):223-8.
To observe the effect of "Xingnao Kaiqiao Zhenfa" (acupuncture technique for restoring consciousness) combined with enriched rehabilitation training on motor function and expression of growth-associated protein-43 (GAP-43) of peri-ischemic cortex in ischemic stroke rats, so as to investigate its mechanism underlying improvement of ischemic stroke.
SD rats were randomly divided into sham operation, model, rehabilitation and comprehensive rehabilitation groups, which were further divided into 3 time-points:7, 14 and 21 d (=6 in each). Cerebral ischemia(CI) model was established by occlusion of the middle cerebral artery with heat-coagulation. The rehabilitation group was treated by enriched rehabilitation training, once a day. The comprehensive rehabilitation group was treated by acupuncture combined with enriched rehabilitation training. Acupuncture was applied to bilateral "Neiguan"(PC 6) and "Shuigou"(GV 26) for 30 min, once a day. The neurological function score, balance-beam walking test and rotating-rod walking test were evaluated at the end of the corresponding treatment time. The expression of GAP-43 in peri-ischemic cortex was detected by immunohistochemistry.
In comparison with the sham operation group, the scores of neurological function, beam walking test and rotating-rod walking test were significantly higher in the model group (<0.01). There were no significant changes in the scores of balance-beam walking and rotating-rod walking tests in the rehabilitation group compared with the model group on day 7 (>0.05). Compared with the model group at the other time points, the scores of neurological function, balance-beam walking test and rotating-rod walking test were significantly lower in the rehabilitation and comprehensive rehabilitation groups (<0.05). Compared with the rehabilitation group, the scores of neurological function, balance-beam walking test and rotating-rod walking test were significantly lower in the comprehensive rehabilitation group (<0.05). In comparison with the sham operation group, the number of GAP-43 positive cells of peri-ischemic cortex was significantly higher in the model group (<0.01). Compared with the model group, the numbers of GAP-43 positive cells of peri-ischemic cortex were significantly increased in the rehabilitation and comprehensive rehabilitation groups (<0.01). The number of GAP-43 positive cells of peri-ischemic cortex in the comprehensive rehabilitation group was significantly higher than that in the rehabilitation group (<0.01).
"Xingnao Kaiqiao Zhenfa" combined with enriched rehabilitation training can promote the recovery of nerve function in ischemic stroke rats, which may be associated with its effect in up-regulating the expression of GAP-43 in the peri-ischemic cortex.
观察醒脑开窍针法结合强化康复训练对缺血性脑卒中大鼠运动功能及缺血周边皮质生长相关蛋白43（GAP - 43）表达的影响，探讨其改善缺血性脑卒中的作用机制。
将SD大鼠随机分为假手术组、模型组、康复组和综合康复组，每组再根据时间点分为7、14和21 d三个亚组（每组6只）。采用热凝法闭塞大脑中动脉建立脑缺血（CI）模型。康复组给予强化康复训练，每天1次。综合康复组采用针刺结合强化康复训练。针刺双侧内关（PC 6）、水沟（GV 26）30 min，每天1次。在相应治疗时间结束时评估神经功能评分、平衡木行走试验和转棒行走试验。采用免疫组织化学法检测缺血周边皮质GAP - 43的表达。
与假手术组相比，模型组神经功能评分、平衡木行走试验评分和转棒行走试验评分显著升高（P < 0.01）。康复组在第7天时平衡木行走试验评分和转棒行走试验评分与模型组相比无显著变化（P > 0.05）。在其他时间点，与模型组相比，康复组和综合康复组神经功能评分、平衡木行走试验评分和转棒行走试验评分显著降低（P < 0.05）。与康复组相比，综合康复组神经功能评分、平衡木行走试验评分和转棒行走试验评分显著降低（P < 0.05）。与假手术组相比，模型组缺血周边皮质GAP - 43阳性细胞数显著增多（P < 0.01）。与模型组相比，康复组和综合康复组缺血周边皮质GAP - 43阳性细胞数显著增加（P < 0.01）。综合康复组缺血周边皮质GAP - 43阳性细胞数显著高于康复组（P < 0.01）。
醒脑开窍针法结合强化康复训练可促进缺血性脑卒中大鼠神经功能的恢复，可能与其上调缺血周边皮质GAP - 43表达有关。
