Department of Developmental Biology and Genetics, National Center for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
The Centre for Molecular Life Sciences, Biozentrum, Basel, Switzerland.
Elife. 2017 Oct 26;6:e30107. doi: 10.7554/eLife.30107.
Work on genetic model systems such as and mouse has shown that the fundamental mechanisms of myogenesis are remarkably similar in vertebrates and invertebrates. Strikingly, however, satellite cells, the adult muscle stem cells that are essential for the regeneration of damaged muscles in vertebrates, have not been reported in invertebrates. In this study, we show that lineal descendants of muscle stem cells are present in adult muscle of as small, unfused cells observed at the surface and in close proximity to the mature muscle fibers. Normally quiescent, following muscle fiber injury, we show that these cells express Zfh1 and engage in Notch-Delta-dependent proliferative activity and generate lineal descendant populations, which fuse with the injured muscle fiber. In view of strikingly similar morphological and functional features, we consider these novel cells to be the equivalent of vertebrate muscle satellite cells.
在诸如线虫和小鼠等遗传模式生物系统上的研究表明，在脊椎动物和无脊椎动物中，肌发生的基本机制非常相似。然而，令人惊讶的是，卫星细胞——对于脊椎动物受损肌肉的再生至关重要的成年肌肉干细胞——尚未在无脊椎动物中报道过。在这项研究中，我们表明，肌肉干细胞的直系后代存在于成年的秀丽隐杆线虫肌肉中，它们是作为小的、未融合的细胞观察到的，位于表面附近且与成熟的肌肉纤维紧密相邻。这些细胞通常处于静止状态，在肌肉纤维损伤后，我们表明它们表达 Zfh1 并参与 Notch-Delta 依赖性增殖活性，产生直系后代群体，这些群体与受损的肌肉纤维融合。鉴于其形态和功能上惊人的相似性，我们认为这些新的细胞是秀丽隐杆线虫的肌肉卫星细胞的对应物。
