Seliger E, Blumenauer V, Heins J, Neubert K, Kaltwasser P, Schöneich C, Bergleiter R
Klinik und Poliklinik für Gynäkologie und Geburtshilfe, Martin-Luther-Universität Halle-Wittenberg.
Zentralbl Gynakol. 1988;110(20):1312-4.
Aspirates of ovarian follicles were obtained from patients subjected to gynaecologic surgeries. This is the first report of dipeptidyl-peptidase IV (DP IV) activity in human follicular fluid. This enzyme specifically cleaves regulatory important oligo- and polypeptides. Only from peptides with proline or alanine in position P1 of the N-terminal sequence a dipeptide is split off. DP IV activity was measured with glycyl-L-proline p-Nitroanilide as substrate. Levels in a range of 209.8 +/- 57.4 nmol l-1 s-1 were achieved.
从接受妇科手术的患者身上获取卵巢卵泡抽吸物。这是关于人卵泡液中二肽基肽酶IV（DP IV）活性的首次报告。该酶特异性切割具有重要调节作用的寡肽和多肽。仅从N端序列P1位置含有脯氨酸或丙氨酸的肽中切下一个二肽。以甘氨酰-L-脯氨酸对硝基苯胺为底物测定DP IV活性。测得的水平在209.8±57.4 nmol l-1 s-1范围内。
