Penny William M, Palmer Christopher P
Department of Chemistry and Biochemistry, University of Montana, Missoula, MT, USA.
Electrophoresis. 2018 Mar;39(5-6):844-852. doi: 10.1002/elps.201700308. Epub 2017 Nov 13.
Styrene-maleic acid polymer-bound lipid bilayer nanodiscs have been investigated and characterized by electrokinetic chromatography. Linear solvation energy relationship analysis was employed to characterize the changes in solvation environment of nanodiscs of varied belt to lipid ratio, belt polymer chemistry and molecular weight, and lipid composition. Increases in the lipid to belt polymer ratio resulted in smaller, more cohesive nanodiscs with greater electrophoretic mobility. Nanodisc structures with belt polymers of different chemistry and molecular weight were compared and showed only minor changes in solvent characteristics and selectivity consistent with changes in structure of the lipid bilayer. Seven phospholipid and sphingomyelin nanodiscs of different lipid composition were characterized. Changes in lipid head group structure had a significant effect on bilayer-solute interactions. In most cases, changes in alkyl tail structure had no discernible effect on solvation environment aside from those explained by changes in the gel-liquid transition temperature. Comparison to vesicles of similar lipid composition show only minor differences in solvation environment, likely due to differences in lipid composition and bilayer curvature. Together these results provide evidence that the dominant solute-nanodisc interactions are with the lipid bilayer and that head group chemistry has a greater impact on bilayer-solute interactions than alkyl tail or belt polymer structure. Nanodisc electrokinetic chromatography is demonstrated to allow characterization of solute interactions with lipid bilayers of varied composition.
苯乙烯-马来酸聚合物结合的脂质双层纳米盘已通过电动色谱法进行了研究和表征。采用线性溶剂化能关系分析来表征不同带与脂质比、带聚合物化学性质和分子量以及脂质组成的纳米盘的溶剂化环境变化。脂质与带聚合物比例的增加导致纳米盘更小、更具凝聚性且电泳迁移率更高。比较了具有不同化学性质和分子量的带聚合物的纳米盘结构，结果表明溶剂特性和选择性仅发生了微小变化，这与脂质双层结构的变化一致。对七种不同脂质组成的磷脂和鞘磷脂纳米盘进行了表征。脂质头部基团结构的变化对双层-溶质相互作用有显著影响。在大多数情况下，除了由凝胶-液晶转变温度变化所解释的那些影响外，烷基尾结构的变化对溶剂化环境没有明显影响。与类似脂质组成的囊泡相比，溶剂化环境仅存在微小差异，这可能是由于脂质组成和双层曲率的差异所致。这些结果共同提供了证据，表明主要的溶质-纳米盘相互作用是与脂质双层的相互作用，并且头部基团化学性质对双层-溶质相互作用的影响比烷基尾或带聚合物结构更大。电动色谱法被证明可以用于表征溶质与不同组成的脂质双层之间的相互作用。
