一种基于苯并噻唑衍生物的用于快速顺序检测铜和生物硫醇的简单荧光探针。
A simple fluorescent probe for the fast sequential detection of copper and biothiols based on a benzothiazole derivative.
作者信息
Shen Youming, Zhang Xiangyang, Zhang Chunxiang, Zhang Youyu, Jin Junling, Li Haitao
机构信息
Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Preparation and Application of Environmentally Friendly Materials (Jilin Normal University), Ministry of Education, Changchun 130103, PR China.
Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 15;191:427-434. doi: 10.1016/j.saa.2017.09.069. Epub 2017 Sep 28.
A simple benzothiazole fluorescent chemosensor was developed for the fast sequential detection of Cu and biothiols through modulating the excited-state intramolecular proton transfer (ESIPT) process. The compound 1 exhibits highly selective and sensitive fluorescence "on-off" recognition to Cu with a 1:1 binding stoichiometry by ESIPT hinder. The in situ generated 1-Cu complex can serve as an "on-off" fluorescent probe for high selectivity toward biothiols via Cu displacement approach, which exerts ESIPT recovery. It is worth pointing out that the 1-Cu complex shows faster for cysteins (within 1min) than other biothiols such as homocysteine (25min) and glutathione (25min). Moreover, the compound 1 displays 160nm Stoke-shift for reversibly monitoring Cu and biothiols. In addition, the probe is successfully used for fluorescent cellular imaging. This strategy via modulation the ESIPT state has been used for determination of Cu and Cys with satisfactory results, which further demonstrates its value of practical applications.
开发了一种简单的苯并噻唑荧光化学传感器，用于通过调节激发态分子内质子转移（ESIPT）过程快速顺序检测铜和生物硫醇。化合物1通过ESIPT阻碍以1:1的结合化学计量比表现出对铜的高选择性和灵敏的荧光“开-关”识别。原位生成的1-Cu配合物可作为通过铜置换方法对生物硫醇具有高选择性的“开-关”荧光探针，该方法可实现ESIPT恢复。值得指出的是，1-Cu配合物对半胱氨酸的响应速度比其他生物硫醇如高半胱氨酸（25分钟）和谷胱甘肽（25分钟）更快（在1分钟内）。此外，化合物1具有160nm的斯托克斯位移，可用于可逆监测铜和生物硫醇。此外，该探针已成功用于荧光细胞成像。这种通过调节ESIPT状态的策略已用于测定铜和半胱氨酸，结果令人满意，这进一步证明了其实际应用价值。
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