一种基于铜（II）配合物的荧光化学传感器及其在人尿液中生物硫醇的“裸眼”检测中的应用。
A Copper (II) Ensemble-Based Fluorescence Chemosensor and Its Application in the 'Naked-Eye' Detection of Biothiols in Human Urine.
机构信息
School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China.
Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
出版信息
Sensors (Basel). 2020 Feb 29;20(5):1331. doi: 10.3390/s20051331.
Quick and effective detection of biothiols in biological fluids has gained increasing attention due to its vital biological functions. In this paper, a novel reversible fluorescence chemosensor (-Cu) based on a benzocoumarin-Cu ensemble has been developed for the detection of biothiols (Cys, Hcy and GSH) in human urine. The chemosensing ensemble (-Cu) contains a 2:1 stoichiometry structure between fluorescent ligand and paramagnetic Cu. was found to exclusively bond with Cu ions accompanied with a dramatic fluorescence quenching maximum at 443 nm and an increase of an absorbance band centered at 378 nm. Then, the in situ generated fluorescence sluggish ensemble, -Cu, was successfully used as a chemosensor for the detection of biothiols with a fluorescence "OFF-ON" response modality. Upon the addition of biothiols, the decomplexation of -Cu led to the liberation of the fluorescent ligand, , resulting in the recovery of fluorescence and absorbance spectra. Studies revealed that -Cu possesses simple synthesis, excellent stability, high sensitivity, reliability at a broad pH range and desired renewability (at least 5 times). The practical application of -Cu was then demonstrated by the detection of biothiols in human urine sample.
由于生物硫醇在生物中的重要生物学功能，因此快速有效地检测生物硫醇引起了越来越多的关注。在本文中，基于苯并[ c ]香豆素-Cu 配合物，设计了一种新型的用于检测人尿中生物硫醇（半胱氨酸，同型半胱氨酸和谷胱甘肽）的可逆荧光化学传感器（-Cu）。化学传感器配合物（-Cu）包含荧光配体 和顺磁性 Cu 的 2:1 化学计量结构。发现 仅与 Cu 离子结合，伴随着在 443nm 处的荧光猝灭最大值和在 378nm 处的吸收带的增加。然后，原位生成的荧光迟缓配合物 -Cu 被成功用作生物硫醇的化学传感器，具有荧光“关-开”响应模式。加入生物硫醇后，-Cu 的解络合导致荧光配体 的释放，从而恢复荧光和吸收光谱。研究表明，-Cu 具有简单的合成、优异的稳定性、高灵敏度、在较宽的 pH 范围内的可靠性以及所需的可重复使用性（至少 5 次）。然后通过检测人尿样中的生物硫醇来证明 -Cu 的实际应用。
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