Bonen L, Bird S
Department of Biology, University of Ottawa, Canada.
Gene. 1988 Dec 15;73(1):47-56. doi: 10.1016/0378-1119(88)90311-3.
The nucleotide sequence of the wheat mitochondrial gene for subunit 6 (atp6) of the F1F0 ATPase complex has been determined. Unlike bacterial, chloroplast or animal/fungal mitochondrial atp6 counterparts, which encode proteins of about 230-270 amino acids, the wheat mitochondrial atp6 homologue comprises the latter part of an open reading frame (ORF) of 386 codons. The ATP6 protein may therefore by synthesized with a long N-terminal presequence. This is supported by the finding that the ORF is preceded by a conserved sequence block closely related to ones preceding several other actively transcribed wheat mitochondrial protein-coding genes. The fused upstream ORF is similar in length, but unrelated in sequence, to those preceding the maize and tobacco mitochondrial atp6 genes. In wheat, the atp6 gene is located on a recombinationally active repeated DNA element, whose length of 1.4 kb corresponds approximately to that of the atp6 mRNA. A comparison of the wheat and maize ATP6 sequences reveals unexpectedly high divergence in the region corresponding to the mature N-terminal domain and may reflect mitochondrial DNA rearrangements during atp6 gene evolution in monocotyledonous plants.
已确定小麦线粒体中F1F0 ATP酶复合体亚基6（atp6）的核苷酸序列。与细菌、叶绿体或动物/真菌线粒体中的atp6对应物不同，后者编码约230 - 270个氨基酸的蛋白质，小麦线粒体atp6同源物包含一个386个密码子的开放阅读框（ORF）的后半部分。因此，ATP6蛋白可能是由一个长的N端前序列合成的。这一发现得到了支持，即该ORF之前有一个保守序列块，与其他几个活跃转录的小麦线粒体蛋白质编码基因之前的序列块密切相关。融合的上游ORF在长度上与玉米和烟草线粒体atp6基因之前的ORF相似，但在序列上无关。在小麦中，atp6基因位于一个具有重组活性的重复DNA元件上，其长度为1.4 kb，与atp6 mRNA的长度大致相当。小麦和玉米ATP6序列的比较显示，在对应于成熟N端结构域的区域存在意外的高度差异，这可能反映了单子叶植物atp6基因进化过程中的线粒体DNA重排。
