Bonen L, Bird S, Belanger L
Department of Biology, University of Ottawa, Canada.
Plant Mol Biol. 1990 Nov;15(5):793-5. doi: 10.1007/BF00016131.
The wheat mitochondrial orf25 nucleotide sequence of 576 bp has been determined. Its derived protein sequence shares 88% and 75% amino acid identity with those of maize and tobacco mitochondria, respectively. The wheat and tobacco orf25 sequences lack four inserts, of 6 bp to 36 bp, that are present in the maize homologue. The wheat orf25 gene is actively transcribed and is preceded by a regulatory sequence block very similar to those located upstream of the wheat coxII and atp6 genes. Our observations support the view that orf25 sequences encode a functional polypeptide in plant mitochondria.
已测定了小麦线粒体576 bp的orf25核苷酸序列。其推导的蛋白质序列与玉米和烟草线粒体的蛋白质序列分别具有88%和75%的氨基酸同一性。小麦和烟草的orf25序列缺少玉米同源物中存在的4个6 bp至36 bp的插入片段。小麦orf25基因被活跃转录，并且其前面有一个调控序列块，与位于小麦coxII和atp6基因上游的调控序列块非常相似。我们的观察结果支持这样一种观点，即orf25序列在植物线粒体中编码一种功能性多肽。
