来自黄孢原毛平革菌的多个与木质素酶相关的基因。
Multiple ligninase-related genes from Phanerochaete chrysosporium.
作者信息
Brown A, Sims P F, Raeder U, Broda P
机构信息
Department of Biochemistry and Applied Molecular Biology, University of Manchester, U.K.
出版信息
Gene. 1988 Dec 15;73(1):77-85. doi: 10.1016/0378-1119(88)90314-9.
We have investigated four distinct sequences in genomic DNA of Phanerochaete chrysosporium that show strong homology to a lignin peroxidase cDNA sequence [Tien and Tu: Nature 236 (1987) 520-523, and 328 (1987) 742]. The complete nucleotide sequence of one of these genes is presented here. Comparison of the sequenced gene, LIG1, with the published cDNA sequence reveals the presence of eight short intervening sequences. The open reading frame encodes a polypeptide of 372 amino acids (aa), which includes a 28-aa residue hydrophobic leader sequence. The predicted Mr of the mature protein product is 37,000. Partial sequencing of the other three genes reveals substantial nucleotide and amino acid homology.
我们研究了黄孢原毛平革菌基因组DNA中的四个不同序列，这些序列与木质素过氧化物酶cDNA序列具有很强的同源性[Tien和Tu：《自然》236（1987）520 - 523，以及328（1987）742]。这里给出了其中一个基因的完整核苷酸序列。将测序得到的基因LIG1与已发表的cDNA序列进行比较，发现存在八个短插入序列。开放阅读框编码一个372个氨基酸（aa）的多肽，其中包括一个28个氨基酸残基的疏水前导序列。成熟蛋白质产物的预测相对分子质量为37,000。对其他三个基因的部分测序显示出大量的核苷酸和氨基酸同源性。
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