Stewart P, Cullen D
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
J Bacteriol. 1999 Jun;181(11):3427-32. doi: 10.1128/JB.181.11.3427-3432.1999.
The lignin peroxidases of Phanerochaete chrysosporium are encoded by a minimum of 10 closely related genes. Physical and genetic mapping of a cluster of eight lip genes revealed six genes occurring in pairs and transcriptionally convergent, suggesting that portions of the lip family arose by gene duplication events. The completed sequence of lipG and lipJ, together with previously published sequences, allowed phylogenetic and intron/exon classifications, indicating two main branches within the lip family. Competitive reverse transcription-PCR was used to assess lip transcript levels in both carbon- and nitrogen-limited media. Transcript patterns showed differential regulation of lip genes in response to medium composition. No apparent correlation was observed between genomic organization and transcript levels. Both constitutive and upregulated transcripts, structurally unrelated to peroxidases, were identified within the lip cluster.
黄孢原毛平革菌的木质素过氧化物酶至少由10个密切相关的基因编码。对一组8个lip基因的物理和遗传图谱分析显示,6个基因成对出现且转录方向相反,这表明lip家族的部分基因是通过基因复制事件产生的。lipG和lipJ的完整序列,连同先前发表的序列,进行了系统发育和内含子/外显子分类,表明lip家族内有两个主要分支。采用竞争性逆转录PCR来评估碳限制和氮限制培养基中lip转录本水平。转录本模式显示,lip基因对培养基成分有不同的调控。未观察到基因组组织与转录本水平之间有明显相关性。在lip基因簇中鉴定出了结构上与过氧化物酶无关的组成型和上调转录本。