Zhao Min, Tang Shimiao, Xin Junchi, Liu Dongyan
Medical Research Center, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110000, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4033-4040. doi: 10.3892/etm.2017.5027. Epub 2017 Aug 24.
Redox imbalance is established in various human diseases. Treatment of intestinal epithelial cells with hyperoxia for a prolonged period of time may cause serious effects on redox balance. Secretory component (SC) protein is secreted by intestinal epithelial cells, and has a vital role in mucosal immune systems and intestinal defense. The present study aimed to investigate the influence of reactive oxygen species (ROS) on intestinal epithelial cells and intestinal epithelial SC protein under hyperoxic conditions. Caco-2 cells were treated with increasing concentrations of hydrogen peroxide (HO) or 85% O (hyperoxia) for 24 h. Flow cytometry, immunohistochemistry staining, western blot analysis and reverse transcription-quantitative polymerase chain reaction were performed to detect the expression levels of SC protein. Significantly increased apoptosis and mortality rates were observed in hyperoxia- and HO-treated Caco-2 cells, as compared with the untreated control cells (P<0.05). Protein and mRNA expression levels of SC were significantly increased in hyperoxia- and HO-treated groups, as compared with the control group (P<0.05). During hyperoxia, intestinal epithelial cells were destroyed and ROS levels increased. Therefore, the results of the present study suggested that ROS might have an important role in intestinal injury in hyperoxic environments.
氧化还原失衡在多种人类疾病中均有发生。长时间用高氧处理肠上皮细胞可能会对氧化还原平衡造成严重影响。分泌成分（SC）蛋白由肠上皮细胞分泌，在黏膜免疫系统和肠道防御中发挥着至关重要的作用。本研究旨在探讨活性氧（ROS）在高氧条件下对肠上皮细胞和肠上皮SC蛋白的影响。用不同浓度的过氧化氢（HO）或85%氧气（高氧）处理Caco-2细胞24小时。采用流式细胞术、免疫组织化学染色、蛋白质印迹分析和逆转录-定量聚合酶链反应检测SC蛋白的表达水平。与未处理的对照细胞相比，高氧和HO处理的Caco-2细胞凋亡率和死亡率显著升高（P<0.05）。与对照组相比，高氧和HO处理组SC的蛋白质和mRNA表达水平显著升高（P<0.05）。在高氧期间，肠上皮细胞遭到破坏，ROS水平升高。因此，本研究结果表明，ROS可能在高氧环境下的肠道损伤中发挥重要作用。
