Medical Research Center, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110000, P.R. China.
Department of Orthopaedic Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110000, P.R. China.
Int J Mol Med. 2018 Jan;41(1):322-330. doi: 10.3892/ijmm.2017.3247. Epub 2017 Nov 9.
Long-term therapeutic hyperoxia may exert serious toxic effects on intestinal epithelial cells in vitro and in vivo. The aim of the present study was to investigate the cause of this intestinal injury under conditions of hyperoxia. Caco-2 cells were treated with different concentrations of hydrogen peroxide (H2O2) and 85% hyperoxia for 24 h. higher rates of injury of Caco-2 cells were observed in the hyperoxia and H2O2 groups compared with the control group. The reactive oxygen species (ROS) level of the hyperoxia group was significantly higher compared with that of the 400 µM H2O2 group. The protein and gene levels of RelA, RelB, hypoxia‑inducible factor-1α, tumor necrosis factor-α and apoptosis signal‑regulating kinase 1 were significantly higher in the hyperoxia and H2O2 groups compared with those in the control group. In conclusion, during hyperoxia, intestinal epithelial cells were destroyed and the levels of ROS were increased. Therefore, ROS may play an important role in intestinal injury in a hyperoxic environment.
长期治疗性高氧可能会对体外和体内的肠上皮细胞产生严重的毒性作用。本研究旨在探讨高氧条件下这种肠损伤的原因。Caco-2 细胞用不同浓度的过氧化氢 (H2O2) 和 85%高氧处理 24 小时。与对照组相比，高氧和 H2O2 组的 Caco-2 细胞损伤率更高。与 400μM H2O2 组相比，高氧组的活性氧 (ROS) 水平明显更高。与对照组相比，高氧和 H2O2 组的 RelA、RelB、缺氧诱导因子-1α、肿瘤坏死因子-α 和凋亡信号调节激酶 1 的蛋白和基因水平明显升高。综上所述，在高氧环境中，肠上皮细胞被破坏，ROS 水平升高。因此，ROS 在高氧环境中的肠道损伤中可能发挥重要作用。
